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Mouse Monoclonal Estrogen Inducible Protein pS2 antibody. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TFF1.

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Images

Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118), expandable thumbnail
  • Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TFF1. Database link P04155

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytProtein ArrayICC/IFIHC-P
Human
Tested
Expected
Tested
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

3 products for Alternative Product

Target data

Function

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Alternative names

Recommended products

Mouse Monoclonal Estrogen Inducible Protein pS2 antibody. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TFF1.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human TFF1. Database link P04155
Clone number
TFF1/2133
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from bioreactor concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Estrogen Inducible Protein pS2 also known as Trefoil Factor 1 (TFF1) is a small protein with a mass of about 6.6 kDa. It is mainly expressed in the mucosa of the stomach and other tissues responsive to estrogen. pS2 functions mechanically as part of the trefoil factor family known for its role in mucosal protection and repair. pS2 has a distinctive three-loop structure which is key to its interaction with other molecules and substrates in the cellular environment.

Biological function summary

This trefoil factor plays a significant role in the process of epithelial regeneration and maintaining gastrointestinal tract integrity. It is not known to be part of a complex but works through interaction with various growth factors and cytokines. Its expression is regulated by estrogen making pS2 significant in estrogen-responsive tissues. These interactions contribute to maintaining the stability of epithelial lining aiding in cellular migration and wound healing.

Pathways

PS2 influences cellular signaling involved in the gastric mucosal defense mainly interacting with the Epidermal Growth Factor (EGF) pathway. It modulates the pathway's effects on cell proliferation and migration. Its role is linked to the mitigation of tissue damage indicating a connection with other proteins like the EGF receptor contributing to the repair and defense mechanisms of epithelial tissues.

Associated diseases and disorders

PS2 has gained attention in breast cancer research. Its expression correlates with hormone receptor-positive breast cancer making it a potential biomarker for the disease. Additionally aberrant expression levels of pS2 might relate to gastric cancer where it plays a role in tumor progression and response to therapy. pS2 is closely connected to estrogen receptor proteins which guide its involvement in these cancers influencing prognosis and treatment strategies.

Product promise

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5 product images

  • Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118), expandable thumbnail

    Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118)

    Flow Cytometric analysis of PFA-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Inducible Protein pS2 using ab268118 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Inducible Protein pS2 using ab268118 at 2 μg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118)

    Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Green). Nucleus is labeled with Reddot (Red).

  • Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118), expandable thumbnail

    Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118)

    Protein Array containing more than 19,000 full-length human proteins using ab268118.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (ab268118)

    Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Green). Membrane are labeled with phalloidin (Red).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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