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AB268118

Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133]

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(2 Publications)

Mouse Monoclonal Estrogen Inducible Protein pS2 antibody. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TFF1.

View Alternative Names

BCEI, PS2, TFF1, Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, Protein pS2, hP1.A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)

Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Green). Nucleus is labeled with Reddot (Red).

Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)

Flow Cytometric analysis of PFA-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Inducible Protein pS2 using ab268118 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Inducible Protein pS2 using ab268118 at 2 μg/ml in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)

Immunofluorescence staining of paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells stained for Estrogen Inducible Protein pS2 with ab268118 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Green). Membrane are labeled with phalloidin (Red).

Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)
  • Protein Array

Unknown

Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] (AB268118)

Protein Array containing more than 19,000 full-length human proteins using ab268118.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TFF1/2133

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, Protein Array, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TFF1.

P04155

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Estrogen Inducible Protein pS2 also known as Trefoil Factor 1 (TFF1) is a small protein with a mass of about 6.6 kDa. It is mainly expressed in the mucosa of the stomach and other tissues responsive to estrogen. pS2 functions mechanically as part of the trefoil factor family known for its role in mucosal protection and repair. pS2 has a distinctive three-loop structure which is key to its interaction with other molecules and substrates in the cellular environment.
Biological function summary

This trefoil factor plays a significant role in the process of epithelial regeneration and maintaining gastrointestinal tract integrity. It is not known to be part of a complex but works through interaction with various growth factors and cytokines. Its expression is regulated by estrogen making pS2 significant in estrogen-responsive tissues. These interactions contribute to maintaining the stability of epithelial lining aiding in cellular migration and wound healing.

Pathways

PS2 influences cellular signaling involved in the gastric mucosal defense mainly interacting with the Epidermal Growth Factor (EGF) pathway. It modulates the pathway's effects on cell proliferation and migration. Its role is linked to the mitigation of tissue damage indicating a connection with other proteins like the EGF receptor contributing to the repair and defense mechanisms of epithelial tissues.

PS2 has gained attention in breast cancer research. Its expression correlates with hormone receptor-positive breast cancer making it a potential biomarker for the disease. Additionally aberrant expression levels of pS2 might relate to gastric cancer where it plays a role in tumor progression and response to therapy. pS2 is closely connected to estrogen receptor proteins which guide its involvement in these cancers influencing prognosis and treatment strategies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
See full target information TFF1

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 24:99 PubMed37005656

2023

Morphologically intact airways in lung fibrosis have an abnormal proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy A Herrera,Lewis A Dingle,M Angeles Monetero,Rajamiyer V Venkateswaran,John F Blaikley,Felice Granato,Stella Pearson,Craig Lawless,David J Thornton

JCI insight 8: PubMed36821371

2023

EPIREGULIN creates a developmental niche for spatially organized human intestinal enteroids.

Applications

Unspecified application

Species

Unspecified reactive species

Charlie J Childs,Emily M Holloway,Caden W Sweet,Yu-Hwai Tsai,Angeline Wu,Abigail Vallie,Madeline K Eiken,Meghan M Capeling,Rachel K Zwick,Brisa Palikuqi,Coralie Trentesaux,Joshua H Wu,Oscar Pellón-Cardenas,Charles J Zhang,Ian Glass,Claudia Loebel,Qianhui Yu,J Gray Camp,Jonathan Z Sexton,Ophir D Klein,Michael P Verzi,Jason R Spence
View all publications

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