Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (AB268241)

Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/ml (Green). The secondary antibody is a Goat anti-mouse IgG CF488 conjugate. Membrane are labeled with phalloidin (Red).

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

• Flow Cyt

Unknown

Flow Cytometry - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (AB268241)

Flow cytometric analysis of PFA-fixed MCF7 cells cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/10⁶ cells (Blue) compared with an Isotype control (Red). The secondary antibody was a Goat anti-mouse IgG CF488 conjugate.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (AB268241)

Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling Estrogen Inducible Protein pS2 with ab268118 at 2 μg/ml (Green). The secondary antibody is a Goat anti-mouse IgG CF488 conjugate. Membrane are labeled with phalloidin (Red).

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).

• Protein Array

Unknown

Protein Array - Anti-Estrogen Inducible Protein pS2 antibody [TFF1/2133] - BSA and Azide free (AB268241)

Protein Array containing more than 19,000 full-length human proteins using ab268118.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268118).