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AB3575

Anti-Estrogen Receptor alpha antibody

4

(3 Reviews)

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(46 Publications)

Anti-Estrogen Receptor alpha antibody (ab3575) is a rabbit polyclonal antibody detecting Estrogen Receptor alpha in Western Blot, IHC-P, ICC/IF. Suitable for Human, Rat,.

- Over 40 publications
- Trusted since 2003

View Alternative Names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (AB3575)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (AB3575)

4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (AB3575)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (AB3575)

ab3575 at a dilution of 5 μg/ml staining ER alpha in rat prostate tissue sections.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ESR1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P03372

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Estrogen Receptor alpha antibody (ab3575) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat, samples.

What is the molecular weight of Estrogen Receptor alpha?
Anti-Estrogen Receptor alpha (ab3575) specifically detects a band for Estrogen Receptor alpha (UniProt: P03372) at a molecular weight of 68kDa.

Trusted by the scientific community
Anti-Estrogen Receptor alpha (ab3575) was first used in a scientific publication in 2003 and has been cited over 40 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed : 17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity).. Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed : 21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed : 10970861). Binds to ERE and inhibits isoform 1 (PubMed : 10970861).
See full target information ESR1
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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114552 PubMed39068660

2024

ASCT2 is a major contributor to serine uptake in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly O Conger,Christopher Chidley,Mete Emir Ozgurses,Huiping Zhao,Yumi Kim,Svetlana E Semina,Philippa Burns,Vipin Rawat,Lina Lietuvninkas,Ryan Sheldon,Issam Ben-Sahra,Jonna Frasor,Peter K Sorger,Gina M DeNicola,Jonathan L Coloff

Poultry science 103:103669 PubMed38603931

2024

Influence of sex hormones on the aggressive behavior during peck order establishment and stabilization in meat and egg type chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Jou-Ching Lin,Courtney Lynd Daigle,Pin-Chi Tang,Chien-Kai Wang

Science advances 10:eadj1354 PubMed38266095

2024

CYP46A1-mediated cholesterol turnover induces sex-specific changes in cognition and counteracts memory loss in ovariectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

María Latorre-Leal,Patricia Rodriguez-Rodriguez,Luca Franchini,Orestis Nikolidakis,Makrina Daniilidou,Ljerka Delac,Mukesh K Varshney,Luis E Arroyo-García,Francesca Eroli,Bengt Winblad,Kaj Blennow,Henrik Zetterberg,Miia Kivipelto,Manuela Pacciarini,Yuqin Wang,William J Griffiths,Ingemar Björkhem,Anna Matton,Ivan Nalvarte,Paula Merino-Serrais,Angel Cedazo-Minguez,Silvia Maioli

iScience 26:107568 PubMed37622003

2023

Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jason A Rizo,Kimberly M Davenport,Wipawee Winuthayanon,Thomas E Spencer,Andrew M Kelleher

International journal of molecular sciences 24: PubMed36982683

2023

Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland.

Applications

Unspecified application

Species

Unspecified reactive species

Koraljka Hat,Ana Planinić,Davor Ježek,Snježana Kaštelan

Scientific reports 13:2940 PubMed36808140

2023

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Chenzhong Jin,Yuzhen Yi,Yihong Hu,Xuefeng Han,Zhiliang Tan,Zheng Wang,Jinhe Kang

Journal of medicinal chemistry 66:1210-1220 PubMed36602888

2023

Development of a Novel F-Labeled Probe for PET Imaging of Estrogen Receptor β.

Applications

Unspecified application

Species

Unspecified reactive species

Yujing Zhou,Peng Lei,Jiaxin Han,Zhiming Wang,Aiyan Ji,Yuyang Wu,Lingling Zheng,Xiaoqing Zhang,Chunrong Qu,Jian Min,Weiliang Zhu,Zhijian Xu,Xingdang Liu,Hao Chen,Zhen Cheng

Journal of clinical ultrasound : JCU 51:195-202 PubMed36539919

2022

Application of dual-source computed tomography in the diagnosis of thyroid cancer and evaluation of biological behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Wu,Xuewen Fang,Jianming Li,An Zhang,Yujian Zou,Xiaolin Zheng

International journal of molecular sciences 23: PubMed36499085

2022

Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Datu Agasi Mohd Kamal,Siti Fatimah Ibrahim,Azizah Ugusman,Mohd Helmy Mokhtar

Toxins 14: PubMed36355982

2022

Zearalenone Promotes Uterine Development of Weaned Gilts by Interfering with Serum Hormones and Up-Regulating Expression of Estrogen and Progesterone Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Song,Xuemei Zhou,Xiangming Ma,Yanping Jiang,Weiren Yang,Faxiao Liu,Mei Liu,Libo Huang,Shuzhen Jiang
View all publications
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