AB252794

Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free

Name: Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794) | Abcam
Brand: Abcam Limited
SKU: AB252794
Price: 905 USD
Availability: InStock
Rating: 1 (1 reviews)

BOND RX™ Validated
Recombinant
What is this?

(1 Review)

(1 Publication)

Mouse Recombinant Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma and mouse breast tissues labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1 : 5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).

Immunohistochemical analysis of paraffin-embedded mouse breast labeling Estrogen Receptor with ab241557 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab241557 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1 : 5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

All lanes:

Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] (<a href='/en-us/products/primary-antibodies/estrogen-receptor-alpha-antibody-cret94d-ab241557'>ab241557</a>) at 1/1000 dilution

Lane 1:

Human breast cancer tissue lysates at 20 µg

Lane 2:

Mouse breast tissue lysates at 20 µg

Lane 3:

Mouse ovary tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>)

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Unconjugated

Anti-Estrogen Receptor alpha antibody [CRET94D]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CRET94D

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

ab252794 is the carrier-free version of ab241557.

This product has switched from a hybridoma to recombinant production method on 7th December 2020.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.

Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed : 17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity).. Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed : 21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed : 10970861). Binds to ERE and inhibits isoform 1 (PubMed : 10970861).

See full target information ESR1

Alternative Names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current protocols 4:e1030 PubMed38923763

2024

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein-Ligand Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Dana M Burris,Samuel W Gillespie,Emma Joy Campbell,S Nick Ice,Vikas Yadav,William D Picking,Christian L Lorson,Kamal Singh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (AB252794)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise