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Rabbit Recombinant Monoclonal Estrogen Receptor alpha antibody. Suitable for ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, ChIC/CUT&RUN-seq and reacts with Human, Mouse, Rat samples. Cited in 167 publications.
Alternative names=Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, NR3A1, ESR1, ESR
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | ChIC/CUT&RUN-seq | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Expected | Expected | Expected |
| Rat | Expected | Tested | Tested | Expected | Expected | Expected |
Images
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg chromatin for 25 µg chromatin | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200 | Notes - |
Species Mouse | Dilution info 1/200 | Notes - |
Species Human | Dilution info 1/200 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. We recommend to use a 30 min blocking step(1XPBS/10%goat serum/0,3M Glycin). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/5000 | Notes For unpurified, use 1/50 - 1/100. The antibody failed to show good IHC signal on mouse and rat tissue sections when applied using our IHC testing conditions. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Recommended products
Rabbit Recombinant Monoclonal Estrogen Receptor alpha antibody. Suitable for ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, ChIC/CUT&RUN-seq and reacts with Human, Mouse, Rat samples. Cited in 167 publications.
Alternative names=Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, NR3A1, ESR1, ESR
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
E115
- Purification technique
Affinity purification Protein A
- Specificity
Expression levels of ER alpha protein vary with sample type. This antibody is unsuitable to test ovary and the tissues with low expression level of Estrogen Receptor alpha, such as kidney, lung and brain, in western blot. And it failed to show good IHC signal on mouse and rat tissue sections when using our IHC testing conditions. For our in-house testing we tested the antibody on a mouse tissue array (breast, spleen, lung, stomach, muscle, pancreas, liver, colon, brain, kidney).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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