Name: Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free
SKU: ab167610
Rating: 5 (2 reviews)

Rabbit Recombinant Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-Fr, IHC-P, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 1 publication.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (AB167610), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	Flow Cyt (Intra)	IHC-Fr	IHC-P	ChIC/CUT&RUN-seq
Human	Tested	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

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15 products for Alternative Product

3 products for Alternative Version

Target data

See full target information ESR1

Function

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity). Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).

Alternative names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR4097
Purification technique: Affinity purification Protein A
Specificity: Expression levels of ER alpha protein vary with sample type.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab167610 is the carrier-free version of Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.

Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

Associated diseases and disorders

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling Estrogen Receptor alpha with purified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Clone EPR4097 (ab167610) has been successfully conjugated by Abcam. This image was generated using Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] ab205851 for protocol details.
Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] ab205851 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] ab205851 at a 1/50 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min)
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Clone EPR4097 (ab167610) has been successfully conjugated by Abcam. This image was generated using Anti-Estrogen Receptor alpha antibody [EPR4097] (Alexa Fluor® 488). Please refer to ab205850 for protocol details.
ab205850 staining Estrogen Receptor alpha in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205850 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling Estrogen Receptor alpha (green) with purified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 staining Estrogen Receptor alpha in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast ductal infiltrating carcinoma tissue labelling Estrogen Receptor alpha with unpurified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human normal tonsil tissue. Unpurified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 shows negative staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human colonic adenocarcinoma tissue. Unpurified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 shows negative staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human lung adenocarcinoma tissue. Unpurified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 shows negative staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human cervical carcinoma tissue. Unpurified Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 shows negative staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
ChIC/CUT&RUN sequencing - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 MCF7 (Human breast adenocarcinoma epithelial cell) cells treated with phenol red free medium and 5% charcoal stripped FBS for 3 days than treated with β-estradiol (10 nM 45 min) and 5 µg of Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 [EPR4097]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 MCF7 (Human breast adenocarcinoma epithelial cell) cells treated with phenol red free medium and 5% charcoal stripped FBS for 3 days than treated with β-estradiol (10 nM 45 min) and 5 µg of Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 [EPR4097]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 MCF7 (Human breast adenocarcinoma epithelial cell) cells treated with phenol red free medium and 5% charcoal stripped FBS for 3 days than treated with β-estradiol (10 nM 45 min) and 5 µg of Anti-Estrogen Receptor alpha antibody [EPR4097] ab108398 [EPR4097]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."