Rabbit Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for mIHC, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
pH: 7.2 - 7.4
Constituents: PBS
mIHC | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
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Human | Tested | Expected | Tested | Tested | Tested |
Mouse | Predicted | Not recommended | Predicted | Predicted | Predicted |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Pig | Predicted | Not recommended | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/200 | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes - |
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Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity). Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).
ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Rabbit Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for mIHC, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
pH: 7.2 - 7.4
Constituents: PBS
ab187260 is the carrier-free version of Anti-Estrogen Receptor alpha antibody [SP1] ab16660.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.
ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.
ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Estrogen Receptor alpha immunofluorescence staining of MCF7 cells using rabbit anti-Estrogen Receptor alpha antibody
Anti-Estrogen Receptor alpha antibody [SP1] ab16660 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/250 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594) at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
This image was generated using the hybridoma version of the product.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Clone SP1 (ab187260) has been successfully conjugated by Abcam. This image was generated using Anti-Estrogen Receptor alpha antibody [SP1] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [SP1] ab267512 for protocol details.
IHC image of Estrogen Receptor alpha staining in a section of formalin-fixed paraffin-embedded normal human breast*.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [SP1] ab267512 at 1/100 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Estrogen Receptor alpha immunohistochemistry staining of human cervix using rabbit anti-Estrogen Receptor alpha antibody
IHC image of Anti-Estrogen Receptor alpha antibody [SP1] ab16660 staining Estrogen Receptor alpha in normal human cervix formalin-fixed paraffin-embedded tissue sections* performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6 epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Estrogen Receptor alpha antibody [SP1] ab16660 1/250 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
This image was generated using the hybridoma version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank supported by the NIHR Cambridge Biomedical Research Centre
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Estrogen Receptor alpha immunohistochemistry staining of human ovarian adenocarcinoma using rabbit anti-Estrogen Receptor alpha antibody
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Estrogen Receptor alpha using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Estrogen Receptor alpha flow cytometry staining of MCF7 cells using rabbit anti-Estrogen Receptor alpha antibody
Intracellular flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling Estrogen Receptor alpha with purified Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/200 dilution (1.06 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).This image was generated using the hybridoma version of the product.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660).
Estrogen Receptor alpha immunohistochemistry staining of human breast carcinoma using rabbit anti-Estrogen Receptor alpha antibody
Formalin-fixed paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Estrogen Receptor alpha immunohistochemistry staining of human breast ductal carcinoma using rabbit anti-Estrogen Receptor alpha antibody
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Estrogen Receptor alpha using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Formalin-fixed, paraffin-embedded human breast tissue stained for Estrogen Receptor alpha using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 at 1/200 dilution in immunohistochemical analysis.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Estrogen Receptor alpha immunohistochemistry staining of human breast carcinoma using rabbit anti-Estrogen Receptor alpha antibody
Human breast carcinoma stained with Anti-Estrogen Receptor alpha antibody [SP1] ab16660.
This image was generated using the hybridoma version of the product.
.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Estrogen Receptor alpha antibody [SP1] ab16660)
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling ER alpha with ab187260 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 min with ULTRA cell conditioning solution (CC1 pH 8.5). ab187260 anti ER alpha antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using Anti-Estrogen Receptor alpha antibody [SP1] ab16660, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling Estrogen Receptor (ER) with Anti-Estrogen Receptor alpha antibody [SP1] ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma.
Panel B: anti-PR stained on no cells.
Panel C: anti-HER2 stained on no cells.
Panel D: anti-ER stained on no cells.
The section was incubated in three rounds of staining: in the order of Anti-Progesterone Receptor antibody [SP2] ab16661 for 30 mins, then Anti-Estrogen Receptor alpha antibody [SP1] ab16660 and Anti-ErbB2 / HER2 antibody [SP101] ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Estrogen Receptor alpha antibody [SP1] ab16660, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling Estrogen Receptor (ER) with Anti-Estrogen Receptor alpha antibody [SP1] ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma.
Panel B: anti-PR stained on nucleus of cancer cells.
Panel C: anti-HER2 stained on membrane of cancer cells.
Panel D: anti-ER stained on nucleus of cancer cells.
The section was incubated in three rounds of staining: in the order of Anti-Progesterone Receptor antibody [SP2] ab16661 for 30 mins, then Anti-Estrogen Receptor alpha antibody [SP1] ab16660 and Anti-ErbB2 / HER2 antibody [SP101] ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Estrogen Receptor alpha antibody [SP1] ab16660, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling Estrogen Receptor (ER) with Anti-Estrogen Receptor alpha antibody [SP1] ab16660, at a 1/200 dilution ( 0.07 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.
Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland.
Panel B: anti-PR stained on nucleus of some ductal cells.
Panel C: anti-HER2 stained on no cells.
Panel D: anti-ER stained on nucleus of some ductal cells.
The section was incubated in three rounds of staining: in the order of Anti-Progesterone Receptor antibody [SP2] ab16661 for 30 mins, then Anti-Estrogen Receptor alpha antibody [SP1] ab16660 and Anti-ErbB2 / HER2 antibody [SP101] ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Estrogen Receptor alpha antibody [SP1] ab16660, the same antibody clone in a different buffer formulation.
Tissue Microarrays stained for Anti-Estrogen Receptor alpha antibody [SP1] using Anti-Estrogen Receptor alpha antibody [SP1] ab16660 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The section was incubated with Anti-Estrogen Receptor alpha antibody [SP1] ab16660 for 10 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins.
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