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Mouse Monoclonal Estrogen Receptor alpha antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ESR1 aa 300 to C-terminus.


Images

Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (AB16460), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.08% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ESR1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P03372

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Predicted
Hamster
Predicted
Pig
Predicted
Rabbit
Predicted
Sheep
Predicted

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig
Dilution info
-
Notes

-

Associated Products

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Target data

Function

The protein expressed by the gene ESR1 functions as a nuclear hormone receptor involved in regulating eukaryotic gene expression, affecting cellular proliferation and differentiation in target tissues. It mediates ligand-dependent nuclear transactivation either through direct homodimer binding to estrogen response elements (ERE) or by associating with other transcription factors like AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3, allowing ERE-independent signaling. Ligand binding triggers a conformational change, facilitating the interaction with coactivator complexes through LXXLL motifs. This interaction leads to mutual transrepression with NF-kappa-B in a cell-type-specific manner, reducing NF-kappa-B DNA-binding activity and transcription from the IL6 promoter while displacing coregulators like RELA/p65. It can antagonistically or synergistically interact with NF-kappa-B for transcription activation, involving recruitment to adjacent response elements, working with CREBBP. Additionally, it activates the transcription of TFF1 and mediates membrane-initiated signaling through kinase cascades. It's crucial for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 specifically activates NOS3 and endothelial nitric oxide production, while isoforms lacking certain functional domains modulate transcriptional activity by competitive ligand or DNA binding and heterodimerization with the full-length receptor, capable of binding to ERE and inhibiting isoform 1. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Recommended products

Mouse Monoclonal Estrogen Receptor alpha antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ESR1 aa 300 to C-terminus.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human ESR1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P03372
Clone number
TE111.5D11
Purification technique
Affinity purification Protein A/G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.

Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

Associated diseases and disorders

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.

Product promise

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1 product image

  • Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460), expandable thumbnail

    Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)

    ab16460 at 1μg/ml detecting Estrogen Recptor from MCF-7 cells by Western blot.

    All lanes: Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)

    Predicted band size: 66 kDa

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Product protocols

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