Rabbit Polyclonal Estrogen Receptor alpha phospho S167 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ESR1 phospho S167 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ESR1 phospho S167 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P03372
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity). Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).
Alternative names
ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Recommended products
Rabbit Polyclonal Estrogen Receptor alpha phospho S167 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ESR1 phospho S167 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ESR1 phospho S167 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P03372
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab131105 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
- Biological function summary
ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.
- Pathways
ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.
- Associated diseases and disorders
ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Western blot - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105)
All lanes: Western blot - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105) at 1/500 dilution
Lane 1: MCF7 cell extract with blocking peptide
Lane 2: MCF7 cell extract
Predicted band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S167) antibody (ab131105)
Immunofluorescence analysis of methanol-fixed MCF cells labelling Estrogen Receptor alpha (phospho S167) with ab131105 at 1/100 dilution.
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