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Mouse Monoclonal Estrogen Receptor alpha antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ESR1.

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Images

Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995), expandable thumbnail
  • Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ESR1. Database link P03372

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBProtein ArrayIHC-P
Human
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
0.5-1 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

2 products for Alternative Product

Target data

Function

The protein expressed by the gene ESR1 functions as a nuclear hormone receptor involved in regulating eukaryotic gene expression, affecting cellular proliferation and differentiation in target tissues. It mediates ligand-dependent nuclear transactivation either through direct homodimer binding to estrogen response elements (ERE) or by associating with other transcription factors like AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3, allowing ERE-independent signaling. Ligand binding triggers a conformational change, facilitating the interaction with coactivator complexes through LXXLL motifs. This interaction leads to mutual transrepression with NF-kappa-B in a cell-type-specific manner, reducing NF-kappa-B DNA-binding activity and transcription from the IL6 promoter while displacing coregulators like RELA/p65. It can antagonistically or synergistically interact with NF-kappa-B for transcription activation, involving recruitment to adjacent response elements, working with CREBBP. Additionally, it activates the transcription of TFF1 and mediates membrane-initiated signaling through kinase cascades. It's crucial for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 specifically activates NOS3 and endothelial nitric oxide production, while isoforms lacking certain functional domains modulate transcriptional activity by competitive ligand or DNA binding and heterodimerization with the full-length receptor, capable of binding to ERE and inhibiting isoform 1. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Recommended products

Mouse Monoclonal Estrogen Receptor alpha antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ESR1.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human ESR1. Database link P03372
Clone number
ESR1/1935
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Estrogen Receptor (ER) also known by its alternate names NR3A1 (nuclear receptor subfamily 3 group A member 1) is a nuclear hormone receptor that binds estrogens such as the 17 beta estradiol molecule. Its molecular mass is approximately 66 kDa. This receptor locates mainly in the cytoplasm and nucleus of cells within tissues such as the breast uterus and bone. The ER exists in two primary subtypes ER alpha and ER beta which differ in their tissue distribution and functional roles.

Biological function summary

The Estrogen Receptor functions as a transcription factor that regulates gene expression when it binds to its ligand which is commonly estradiol. Upon binding 17 beta estradiol the receptor dimerizes and interacts with specific DNA sequences to influence transcription. It significantly regulates genes responsible for cell growth differentiation and reproductive functions. The estradiol chemical structure allows for specific interactions with ER altering the transcriptional regulation in a ligand-dependent manner. Though functioning mainly as a monomer ER can form heterodimers with other nuclear receptors affecting its biological role.

Pathways

The Estrogen Receptor is an important player in the genomic signaling pathway where it modulates the expression of numerous target genes by direct DNA binding. It also participates in the non-genomic signaling pathway where it involves the activation of second messengers and kinases. ER closely associates with important proteins such as coactivators and corepressors in these pathways. Estradiol chemical formula interactions facilitate molecular events involving ER-alpha to influence components of cell proliferation and survival.

Associated diseases and disorders

The Estrogen Receptor is intricately linked to breast cancer and osteoporosis. Dysfunction in ER signaling promotes the proliferation of breast cancer cells often requiring targeted therapies that inhibit its action. In osteoporosis ER activity impacts bone density and turnover through regulation of osteoclasts and osteoblasts. The receptor's interaction with growth factor receptors like HER2 in breast cancer further highlights its significance in disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995), expandable thumbnail

    Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995)

    All lanes: Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995) at 1 µg/mL

    All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995)

    Formalin-fixed, paraffin-embedded human Breast Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/mL in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995)

    Formalin-fixed, paraffin-embedded human Endometrial Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/mL in immunohistochemical analysis.

  • Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995), expandable thumbnail

    Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995)

    ab237995 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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