AB187291

Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Name: Anti-Estrogen Receptor beta 1 antibody [ERb455] (ab187291) | Abcam
Brand: Abcam Limited
SKU: AB187291
Price: 420 USD
Availability: InStock
Rating: 3 (7 reviews)

(7 Reviews)

(11 Publications)

Mouse Monoclonal ESR2 antibody. C-terminal. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ESR2.

View Alternative Names

ESTRB, NR3A2, ESR2, Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2

3 Images

Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.

Lab

Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Western blot : Anti-ESR2 antibody [ERb455] (ab187291) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab187291 was shown to bind specifically to ESR2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution. We were not able to detect the endogenous ESR2 due to low target expression and sensitivity limit of the antibody. Further optimization might be needed for detecting endogenous ESR2.

All lanes:

Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 1 µg/mL

Lane 1:

HEK-293T overexpressing ERS2-myc-His tag cell lysate at 20 µg

Lane 2:

WT HEK293T Mock transfection cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 60 kDa

false

578 PE

PE Anti-Estrogen Receptor beta antibody [ERb455]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ERb455

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ESR2. The exact immunogen used to generate this antibody is proprietary information.

Q92731

Specificity

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab187291 was purified from Bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The estrogen receptor beta 1 also known as ERβ or NR3A2 is a type of nuclear hormone receptor. It plays a role in modulating gene expression in response to estrogens. ERβ has an approximate mass of 59 kDa and is expressed in a variety of tissues including the ovary prostate and brain. It differs from estrogen receptor alpha (ERα) in terms of distribution and function within the body.

Biological function summary

Estrogen receptor beta 1 functions as a transcription factor modulating various gene expressions in response to estrogen binding. It forms a complex with coactivators or corepressors to regulate its activity. ERβ is known for balancing the proliferative effects that ERα often promotes thereby maintaining cellular homeostasis in various tissues. It contributes to processes like bone density maintenance lipid metabolism and neural differentiation.

Pathways

The activity of estrogen receptor beta 1 is integrated into the estrogen signaling pathway. It interacts with proteins such as ERα and other nuclear receptors to mediate hormonal responses. This receptor is also part of the MAPK pathway which is important for cell proliferation and differentiation. The balance between ERβ and ERα signaling pathways is key for cellular response to estrogens influencing various physiological outcomes.

Estrogen receptor beta 1 has associations with conditions such as breast cancer and osteoporosis. A decreased ratio of ERβ to ERα often links to more aggressive forms of breast cancer where ERβ acts as a counterbalance to the proliferative signals of ERα. In osteoporosis ERβ’s role in bone density can impact disease progression especially when estrogen levels fluctuate post-menopause. Understanding the balance between ERβ and ERα in these diseases could be useful in developing targeted ERβ activators or inhibitors as therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed : 20074560).. Isoform 2. Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

See full target information ESR2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1602579 PubMed40612952

2025

Expression patterns of estrogen and androgen receptors in NSCLC patients according to the PD-L1 profile.

Applications

Unspecified application

Species

Unspecified reactive species

Vianey Rodriguez-Lara,Gala Cortés-Ramírez,Itzel Amayrani Angeles-Torres,Jeronimo Rodriguez-Cid,Sally María Luisa Pedraza-Reyes,Maria Rosa Avila-Costa,José Luis Ordoñez-Librado,Marco Cerbón

International journal of molecular sciences 26: PubMed40331991

2025

Expression of Estrogen Receptors in Main Immune Organs in Sheep During Early Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yang,Yaqi Zhang,Zhihong Cao,Zhouyuan Li,Leying Zhang,Ling Yang

The world journal of men's health 43:980-991 PubMed39843179

2025

Expression Rates of Sex Hormone Receptors with Their Clinical Correlates in Gastric Cancer Patients and Normal Controls.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghoon Choi,Nayoung Kim,Ji Hyun Park,Chin-Hee Song,Hyeon Jeong Oh

Toxics 12: PubMed39330555

2024

Bisphenol A Disrupts Ribosome Function during Ovarian Development of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Ma,Yongjie Wang,Weiqi Li,Kaiyue Wang,Shilei Zhang

Proceedings of the National Academy of Sciences of the United States of America 121:e2306814121 PubMed38513102

2024

Activation of ERβ hijacks the splicing machinery to trigger R-loop formation in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Wang,Muya Tang,Peidong Zhang,Kailin Yang,Liang Huang,Mengrui Wu,Qiuhong Shen,Jing Yue,Wei Wang,Yanqiu Gong,Margaret Warner,Lunzhi Dai,Haihuai He,Zhengnan Yang,Jan-Ake Gustafsson,Shengtao Zhou

eNeuro 9: PubMed35697512

2022

Ovarian Hormones Regulate Nicotine Consumption and Accumbens Glutamatergic Plasticity in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Erin E Maher,Zachary A Kipp,Jonna M Leyrer-Jackson,Shailesh Khatri,Emma Bondy,Genesee J Martinez,Joshua S Beckmann,Terry D Hinds,Heather A Bimonte-Nelson,Cassandra D Gipson

Journal of neuroinflammation 19:164 PubMed35729568

2022

Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Xu,Wanli Xie,Yiqi Feng,Yanting Wang,Xia Li,Jie Liu,Yue Xiong,Yuyao He,Lu Chen,Guoyang Liu,Qingping Wu

NPJ Regenerative medicine 6:26 PubMed34021161

2021

Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning.

Applications

Unspecified application

Species

Unspecified reactive species

Arvind Manikantan Padma,Laura Carrière,Frida Krokström Karlsson,Edina Sehic,Sara Bandstein,Tom Tristan Tiemann,Mihai Oltean,Min Jong Song,Mats Brännström,Mats Hellström

Frontiers in oncology 10:603883 PubMed33585221

2021

Estrogen Receptor 1 Expression Patterns Have Different Effects on Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors' Treatment Response in Epidermal Growth Factor Receptor Mutant Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Zhang,Meng Tian,Jiamao Lin,Jianbo Zhang,Haiyong Wang,Zhenxiang Li

Molecular human reproduction 26:167-178 PubMed31980817

2020

Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.

Applications

Unspecified application

Species

Unspecified reactive species

T T Tiemann,A M Padma,E Sehic,H Bäckdahl,M Oltean,M J Song,M Brännström,M Hellström

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise