Mouse Monoclonal ESR2 antibody. C-terminal. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human, Mouse, Rat, Horse, Pig, Sheep, Monkey samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ESR2.
Images
![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--flow-cytometry-img128210.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)")
![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--flow-cytometry-img140901.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)")
![Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--western-blot-img436329.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (AB187291)")
Publications
Filter by
- eNeuro 9:2022Ovarian Hormones Regulate Nicotine Consumption and Accumbens Glutamatergic Plasticity in Female Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin E Maher,Zachary A Kipp,Jonna M Leyrer-Jackson,Shailesh Khatri,Emma Bondy,Genesee J Martinez,Joshua S Beckmann,Terry D Hinds,Heather A Bimonte-Nelson,Cassandra D GipsonPubMed 35697512
- Journal of neuroinflammation 19:1642022Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Xu,Wanli Xie,Yiqi Feng,Yanting Wang,Xia Li,Jie Liu,Yue Xiong,Yuyao He,Lu Chen,Guoyang Liu,Qingping WuPubMed 35729568
- NPJ Regenerative medicine 6:262021Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArvind Manikantan Padma,Laura Carrière,Frida Krokström Karlsson,Edina Sehic,Sara Bandstein,Tom Tristan Tiemann,Mihai Oltean,Min Jong Song,Mats Brännström,Mats HellströmPubMed 34021161
- Frontiers in oncology 10:6038832021Estrogen Receptor 1 Expression Patterns Have Different Effects on Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors' Treatment Response in Epidermal Growth Factor Receptor Mutant Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Zhang,Meng Tian,Jiamao Lin,Jianbo Zhang,Haiyong Wang,Zhenxiang LiPubMed 33585221
- Molecular human reproduction 26:167-1782020Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT T Tiemann,A M Padma,E Sehic,H Bäckdahl,M Oltean,M J Song,M Brännström,M HellströmPubMed 31980817
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ESR2. The exact immunogen used to generate this antibody is proprietary information. Database link Q92731
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Expected | Not recommended | Expected | Expected |
| Rat | Expected | Not recommended | Expected | Expected |
| Horse | Expected | Not recommended | Expected | Expected |
| Monkey | Expected | Not recommended | Expected | Expected |
| Pig | Expected | Not recommended | Expected | Expected |
| Sheep | Expected | Not recommended | Expected | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg for 106 Cells | Notes - |
Species Rat | Dilution info 1-2 µg for 106 Cells | Notes - |
Species Horse | Dilution info 1-2 µg for 106 Cells | Notes - |
Species Pig | Dilution info 1-2 µg for 106 Cells | Notes - |
Species Sheep | Dilution info 1-2 µg for 106 Cells | Notes - |
Species Monkey | Dilution info 1-2 µg for 106 Cells | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Monkey, Rat, Pig, Sheep, Horse, Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Monkey, Human, Horse, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
Species Rat | Dilution info 1-2 µg/mL | Notes - |
Species Sheep | Dilution info 1-2 µg/mL | Notes - |
Species Horse | Dilution info 1-2 µg/mL | Notes - |
Species Monkey | Dilution info 1-2 µg/mL | Notes - |
Species Pig | Dilution info 1-2 µg/mL | Notes - |
Target data
Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform 2. Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Alternative names
ESTRB, NR3A2, ESR2, Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2
Recommended products
Mouse Monoclonal ESR2 antibody. C-terminal. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human, Mouse, Rat, Horse, Pig, Sheep, Monkey samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ESR2.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ESR2. The exact immunogen used to generate this antibody is proprietary information. Database link Q92731
- Clone number
- ERb455
- Purification technique
- Affinity purification Protein A
- Specificity
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
- Concentration
- Purification notes
ab187291 was purified from Bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The estrogen receptor beta 1 also known as ERβ or NR3A2 is a type of nuclear hormone receptor. It plays a role in modulating gene expression in response to estrogens. ERβ has an approximate mass of 59 kDa and is expressed in a variety of tissues including the ovary prostate and brain. It differs from estrogen receptor alpha (ERα) in terms of distribution and function within the body.
- Biological function summary
Estrogen receptor beta 1 functions as a transcription factor modulating various gene expressions in response to estrogen binding. It forms a complex with coactivators or corepressors to regulate its activity. ERβ is known for balancing the proliferative effects that ERα often promotes thereby maintaining cellular homeostasis in various tissues. It contributes to processes like bone density maintenance lipid metabolism and neural differentiation.
- Pathways
The activity of estrogen receptor beta 1 is integrated into the estrogen signaling pathway. It interacts with proteins such as ERα and other nuclear receptors to mediate hormonal responses. This receptor is also part of the MAPK pathway which is important for cell proliferation and differentiation. The balance between ERβ and ERα signaling pathways is key for cellular response to estrogens influencing various physiological outcomes.
- Associated diseases and disorders
Estrogen receptor beta 1 has associations with conditions such as breast cancer and osteoporosis. A decreased ratio of ERβ to ERα often links to more aggressive forms of breast cancer where ERβ acts as a counterbalance to the proliferative signals of ERα. In osteoporosis ERβ’s role in bone density can impact disease progression especially when estrogen levels fluctuate post-menopause. Understanding the balance between ERβ and ERα in these diseases could be useful in developing targeted ERβ activators or inhibitors as therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--flow-cytometry-img128210.jpg "Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)")
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.
![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--flow-cytometry-img140901.jpg "Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)")
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.
![Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291), expandable thumbnail](https://content.abcam.com/products/images/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291--western-blot-img436329.jpg "Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)")
Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
Western blot: Anti-ESR2 antibody [ERb455] (ab187291) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab187291 was shown to bind specifically to ESR2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution. We were not able to detect the endogenous ESR2 due to low target expression and sensitivity limit of the antibody. Further optimization might be needed for detecting endogenous ESR2.
All lanes: Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 1 µg/mL
Lane 1: HEK-293T overexpressing ERS2-myc-His tag cell lysate at 20 µg
Lane 2: WT HEK293T Mock transfection cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 60 kDa
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