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Rabbit Recombinant Monoclonal ESR2 antibody. Suitable for Dot, WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (AB323621), expandable thumbnail
  • Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (AB323621), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBICC/IFIHC-P
Human
Expected
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform 2. Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Alternative names

ESTRB, NR3A2, ESR2, Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2

Rabbit Recombinant Monoclonal ESR2 antibody. Suitable for Dot, WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27876-74
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621), expandable thumbnail

    Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: LNCaP, HeLa.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621) at 1/1000 dilution

    Lane 1: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 60 kDa, 36 kDa

    Exposure time: 180s

  • Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621), expandable thumbnail

    Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621)

    Dot blot analysis of Estrogen receptor beta using ab323621 at 1:1000 (0.512 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate 250 ng

    Lane2: 293T cells transfected with a human ESR2 expression vector containing a His-tag, whole cell lysate 50 ng

    Lane3: 293T cells transfected with a human ESR1 expression vector containing a His-tag, whole cell lysate 250 ng

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human ESR1.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 250 ng

    Lane 2: 293T cells transfected with a human ESR2 expression vector containing a His-tag, whole cell lysate at 50 ng

    Lane 3: 293T cells transfected with a human ESR1 expression vector containing a His-tag, whole cell lysate at 250 ng

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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