Anti-Estrogen Related Receptor alpha antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Immunofluorescence analysis of MCF-7 cells fixed in 4% paraformaldehyde at RT for 15 min labelling ERR alpha protein at nucleus using ab137489 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution (Red).

• IP

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Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (AB137489)

ERR alpha was immunoprecipitated from 40μ of HEK-293T whole cell lysate with ab137489 at 1/1000 dilution. Secondary antibody EasyBlot anti-rabbit IgG was used as secondary antibody.
Lane 1 : 40 μg HEK-293T whole cell lysate.
Lane 2 : Control with 2 μg of preimmune rabbit IgG.
Lane 3 : Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody.

7.5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)

Predicted band size: 46 kDa

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• ChIP

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ChIP - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labelling ERR alpha protein at cytoplasm and nucleus with ab137489 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labelling ERR alpha protein at nucleus with ab137489 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

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Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/1000 dilution

Lane 1:

MCF-7 whole cell lysates at 30 µg

Lane 2:

MDA-MB-231 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

• WB

Unknown

Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489)

12% SDS PAGE

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

• WB

Lab

Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489)

Western blot : Anti-ESRRA antibody (ab137489) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137489 was shown to bind specifically to ESRRA. A band was observed at 46 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ESRRA knockout cell line. To generate this image, wild-type and ESRRA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lanes 1 - 4:

Western blot - Human ESRRA knockout MCF7 cell line (ab289292)

Lane 2:

ESRRA knockout MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

ESRRA knockout HAP1 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489)

10% SDS-PAGE

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

All lanes:

Neuro2A whole cell extract at 30 µg

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489)

10% SDS-PAGE

All lanes:

Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

All lanes:

Rat2 whole cell extract at 30 µg

Predicted band size: 46 kDa

false