Rabbit Polyclonal Estrogen Related Receptor alpha antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ESRRA aa 1-50.
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Publications
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- Journal of experimental & clinical cancer research : CR 42:2742023ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPingping Su,Xiaodan Mao,Jincheng Ma,Lixiang Huang,Lirui Yu,Shuting Tang,Mingzhi Zhuang,Zhonglei Lu,Kelvin Stefan Osafo,Yuan Ren,Xinrui Wang,Xite Lin,Leyi Huang,Xiaoli Huang,Elena Ioana Braicu,Jalid Sehouli,Pengming SunPubMed 37864196
- Journal of translational medicine 21:2042023An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuan-Yu Ruan,Li-Xiang Ye,Jian-Song Lin,Hong-Yu Lin,Li-Rui Yu,Cheng-Yan Wang,Xiao-Dan Mao,Shui-Hua Zhang,Peng-Ming SunPubMed 36932403
- International journal of molecular sciences 24:2023ERRα Up-Regulates Invadopodia Formation by Targeting HMGCS1 to Promote Endometrial Cancer Invasion and Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuting Tang,Jincheng Ma,Pingping Su,Huifang Lei,Yao Tong,Liangzhi Cai,Shuxia Xu,Xiaodan Mao,Pengming SunPubMed 36835419
- Journal of experimental & clinical cancer research : CR 41:282022Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodan Mao,Huifang Lei,Tianjin Yi,Pingping Su,Shuting Tang,Yao Tong,Binhua Dong,Guanyu Ruan,Alexander Mustea,Jalid Sehouli,Pengming SunPubMed 35045880
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ESRRA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P11474
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.
Alternative names
ERR1, ESRL1, NR3B1, ESRRA, Steroid hormone receptor ERR1, Estrogen receptor-like 1, Estrogen-related receptor alpha, Nuclear receptor subfamily 3 group B member 1, ERR-alpha
Recommended products
Rabbit Polyclonal Estrogen Related Receptor alpha antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ESRRA aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ESRRA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P11474
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Estrogen Related Receptor Alpha (ERRα) also known as NR3B1 is an orphan nuclear receptor with a molecular mass of approximately 51 kDa. It acts as a transcription factor and influences the expression of genes involved in energy metabolism. ERRα is expressed in numerous tissues such as the liver heart kidney and skeletal muscles. It binds to DNA as a monomer and alters specific gene expression activities. This protein lacks an identified ligand which classifies it as an orphan receptor but it shares structural similarities with estrogen receptors allowing the regulation of gene transcription in several physiological contexts.
- Biological function summary
Estrogen Related Receptor Alpha regulates cellular energy production processes including mitochondrial biogenesis and oxidative phosphorylation. It plays a role in the biological function of energy homeostasis and increases the expression of genes related to metabolic processes. ERRα operates as part of a transcriptional regulatory network and interacts with other nuclear receptors and coactivators to modulate its activity. It often forms complexes with coactivators such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) to exert its effects on mitochondrial function.
- Pathways
ERRα is integral to the PGC-1α signaling pathway and the AMPK (AMP-activated protein kinase) signaling pathway. These pathways are important in regulating energy metabolism and adaptive thermogenesis. ERRα interacts with PGC-1α to enhance mitochondrial function and energy expenditure while AMPK activation affects ERRα transcriptional activity and facilitates cellular energy balance. Additionally ERRα cross-talks with other nuclear receptors like PPAR (peroxisome proliferator-activated receptor) family members which are involved in lipid metabolism and glucose homeostasis.
- Associated diseases and disorders
ERRα has implications in metabolic conditions such as diabetes and obesity. The protein links to diabetes through its role in mitochondrial dysfunction and insulin resistance. Obesity-related studies point out how ERRα affects adaptive thermogenesis and lipid metabolism contributing to energy imbalance. ERRα-PPAR interactions highlight its association in metabolic disorders influencing various aspects of metabolism and disease progression. Understanding ERRα function offers therapeutic potential for targeting metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
ChIP - Anti-Estrogen Related Receptor alpha antibody (ab137489)
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)
10% SDS-PAGE
All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution
All lanes: Neuro2A whole cell extract at 30 µg
Predicted band size: 46 kDa
Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)
10% SDS-PAGE
All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution
All lanes: Rat2 whole cell extract at 30 µg
Predicted band size: 46 kDa
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)
ERR alpha was immunoprecipitated from 40μ of HEK-293T whole cell lysate with ab137489 at 1/1000 dilution. Secondary antibody EasyBlot anti-rabbit IgG was used as secondary antibody.
Lane 1: 40 μg HEK-293T whole cell lysate.
Lane 2: Control with 2 μg of preimmune rabbit IgG.
Lane 3: Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody.7.5% SDS-PAGE
All lanes: Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (ab137489)
Paraffin embedded mouse heart tissue stained for ERR alpha using ab137489 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)
12% SDS PAGE
All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: H1299 whole cell lysate at 30 µg
Predicted band size: 46 kDa
Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)
Western blot: Anti-ESRRA antibody (ab137489) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137489 was shown to bind specifically to ESRRA. A band was observed at 46 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ESRRA knockout cell line. To generate this image, wild-type and ESRRA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/1000 dilution
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lanes 1 - 4: Western blot - Human ESRRA knockout MCF7 cell line (ab289292)
Lane 2: ESRRA knockout MCF7 cell lysate at 20 µg
Lane 3: Wild-type HAP1 cell lysate at 20 µg
Lane 4: ESRRA knockout HAP1 cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 46 kDa
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