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Rabbit Polyclonal Estrogen Related Receptor alpha antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ESRRA aa 1-50.

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Images

ChIP - Anti-Estrogen Related Receptor alpha antibody (AB137489), expandable thumbnail
  • Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489), expandable thumbnail
  • Western blot - Anti-Estrogen Related Receptor alpha antibody (AB137489), expandable thumbnail
  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (AB137489), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (AB137489), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human ESRRA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P11474

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWBIHC-P
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Expected
Tested
Tested
Rat
Expected
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Rat
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Dog, Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

Alternative names

Recommended products

Rabbit Polyclonal Estrogen Related Receptor alpha antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ESRRA aa 1-50.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human ESRRA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P11474
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Estrogen Related Receptor Alpha (ERRα) also known as NR3B1 is an orphan nuclear receptor with a molecular mass of approximately 51 kDa. It acts as a transcription factor and influences the expression of genes involved in energy metabolism. ERRα is expressed in numerous tissues such as the liver heart kidney and skeletal muscles. It binds to DNA as a monomer and alters specific gene expression activities. This protein lacks an identified ligand which classifies it as an orphan receptor but it shares structural similarities with estrogen receptors allowing the regulation of gene transcription in several physiological contexts.

Biological function summary

Estrogen Related Receptor Alpha regulates cellular energy production processes including mitochondrial biogenesis and oxidative phosphorylation. It plays a role in the biological function of energy homeostasis and increases the expression of genes related to metabolic processes. ERRα operates as part of a transcriptional regulatory network and interacts with other nuclear receptors and coactivators to modulate its activity. It often forms complexes with coactivators such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) to exert its effects on mitochondrial function.

Pathways

ERRα is integral to the PGC-1α signaling pathway and the AMPK (AMP-activated protein kinase) signaling pathway. These pathways are important in regulating energy metabolism and adaptive thermogenesis. ERRα interacts with PGC-1α to enhance mitochondrial function and energy expenditure while AMPK activation affects ERRα transcriptional activity and facilitates cellular energy balance. Additionally ERRα cross-talks with other nuclear receptors like PPAR (peroxisome proliferator-activated receptor) family members which are involved in lipid metabolism and glucose homeostasis.

Associated diseases and disorders

ERRα has implications in metabolic conditions such as diabetes and obesity. The protein links to diabetes through its role in mitochondrial dysfunction and insulin resistance. Obesity-related studies point out how ERRα affects adaptive thermogenesis and lipid metabolism contributing to energy imbalance. ERRα-PPAR interactions highlight its association in metabolic disorders influencing various aspects of metabolism and disease progression. Understanding ERRα function offers therapeutic potential for targeting metabolic diseases.

Product promise

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7 product images

  • ChIP - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    ChIP - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

  • Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    10% SDS-PAGE

    All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

    All lanes: Neuro2A whole cell extract at 30 µg

    Predicted band size: 46 kDa

  • Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    10% SDS-PAGE

    All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

    All lanes: Rat2 whole cell extract at 30 µg

    Predicted band size: 46 kDa

  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    ERR alpha was immunoprecipitated from 40μ of HEK-293T whole cell lysate with ab137489 at 1/1000 dilution. Secondary antibody EasyBlot anti-rabbit IgG was used as secondary antibody.
    Lane 1: 40 μg HEK-293T whole cell lysate.
    Lane 2: Control with 2 μg of preimmune rabbit IgG.
    Lane 3: Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody.

    7.5% SDS-PAGE

    All lanes: Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    Paraffin embedded mouse heart tissue stained for ERR alpha using ab137489 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    12% SDS PAGE

    All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Lane 3: H1299 whole cell lysate at 30 µg

    Predicted band size: 46 kDa

  • Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489), expandable thumbnail

    Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)

    Western blot: Anti-ESRRA antibody (ab137489) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137489 was shown to bind specifically to ESRRA. A band was observed at 46 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ESRRA knockout cell line. To generate this image, wild-type and ESRRA knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/1000 dilution

    Lane 1: Wild-type MCF7 cell lysate at 20 µg

    Lanes 1 - 4: Western blot - Human ESRRA knockout MCF7 cell line (ab289292)

    Lane 2: ESRRA knockout MCF7 cell lysate at 20 µg

    Lane 3: Wild-type HAP1 cell lysate at 20 µg

    Lane 4: ESRRA knockout HAP1 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 46 kDa

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