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AB241064

Anti-Estrone antibody [EPPTX-R7-94-2]

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Rabbit Recombinant Monoclonal Estrone antibody. Suitable for cELISA and reacts with Chemical samples.

View Alternative Names

Oestrone

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Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (AB241064)
  • cELISA

Unknown

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (AB241064)

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125 μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (AB241064)
  • cELISA

Unknown

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (AB241064)

ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.

ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Carrier free

    Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R7-94-2

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "0.002-0.0025 µg/mL", "cELISA-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estrone also known as E1 acts as a steroidal estrogen hormone with a molecular weight of approximately 270.4 g/mol. It is commonly expressed in the ovaries adipose tissue and liver. Estrone serves as one of the three major estrogens found in humans alongside estradiol and estriol. As a part of estrogen family it engages in binding to estrogen receptors influencing gene expression and physiological responses in target tissues.
Biological function summary

Estrogen hormones like estrone play significant roles in feminine reproductive health and secondary sexual characteristics. Estrone maintains a balance through conversion to and from other estrogens within the body. It does not function as a complex but influences biological processes such as cell proliferation and tissue growth particularly in reproductive organs. By interacting with cellular mechanisms estrone significantly impacts various physiological responses.

Pathways

Steroid hormones such as estrone integrate into metabolic and reproductive pathways. In the biosynthesis of steroids estrone converts from androstenedione or estradiol through enzymatic actions. The estrogen signaling pathway involves estrone and proteins such as estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ). ERα and ERβ modulate transcriptional activities in various estrogen-responsive genes.

Hormonal imbalances involving estrone can contribute to conditions such as breast cancer and osteoporosis. In breast cancer overexpression of estrone can lead to proliferation of cancerous cells through its interaction with ERα and ERβ proteins. In osteoporosis altered levels of estrone in conjunction with decreased estrogen levels generally result in reduced bone density and mass leading to higher fracture risks. Estrone’s relationship with these disorders highlights its impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Estrone
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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