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AB243564

Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Estrone antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

Oestrone

2 Images
Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)
  • cELISA

Unknown

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)
  • cELISA

Unknown

Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)

ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.

ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R7-94-2

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab243564 is the carrier-free version of ab241064.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estrone also known as E1 acts as a steroidal estrogen hormone with a molecular weight of approximately 270.4 g/mol. It is commonly expressed in the ovaries adipose tissue and liver. Estrone serves as one of the three major estrogens found in humans alongside estradiol and estriol. As a part of estrogen family it engages in binding to estrogen receptors influencing gene expression and physiological responses in target tissues.
Biological function summary

Estrogen hormones like estrone play significant roles in feminine reproductive health and secondary sexual characteristics. Estrone maintains a balance through conversion to and from other estrogens within the body. It does not function as a complex but influences biological processes such as cell proliferation and tissue growth particularly in reproductive organs. By interacting with cellular mechanisms estrone significantly impacts various physiological responses.

Pathways

Steroid hormones such as estrone integrate into metabolic and reproductive pathways. In the biosynthesis of steroids estrone converts from androstenedione or estradiol through enzymatic actions. The estrogen signaling pathway involves estrone and proteins such as estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ). ERα and ERβ modulate transcriptional activities in various estrogen-responsive genes.

Hormonal imbalances involving estrone can contribute to conditions such as breast cancer and osteoporosis. In breast cancer overexpression of estrone can lead to proliferation of cancerous cells through its interaction with ERα and ERβ proteins. In osteoporosis altered levels of estrone in conjunction with decreased estrogen levels generally result in reduced bone density and mass leading to higher fracture risks. Estrone’s relationship with these disorders highlights its impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Estrone
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn
View all publications
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Product promise

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