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AB192402

Anti-ETAA1 antibody

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(2 Publications)

Rabbit Polyclonal ETAA1 antibody. Suitable for WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ETAA1.

View Alternative Names

ETAA16, ETAA1, Ewing's tumor-associated antigen 1, Ewing's tumor-associated antigen 16

1 Images
Western blot - Anti-ETAA1 antibody (AB192402)
  • WB

Supplier Data

Western blot - Anti-ETAA1 antibody (AB192402)

All lanes:

Western blot - Anti-ETAA1 antibody (ab192402) at 1/500 dilution

Lane 1:

MCF7 whole cell lysate

Lane 2:

PC12 whole cell lysate

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ETAA1. The exact immunogen used to generate this antibody is proprietary information.

Q9NY74

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/502", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab192402 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETAA1 also known as Ewing tumor-associated antigen 1 is a protein recognized for its role in DNA replication stress response. It is approximately 92 kDa in mass and ubiquitously expressed in various human tissues. ETAA1 functions at the mechanical level by activating the ATR (ataxia telangiectasia and Rad3-related) kinase an enzyme critical for maintaining genomic stability. This protein contains ATR activators that allow the phosphorylation of various substrates participating in DNA damage repair.
Biological function summary

ETAA1 plays a significant role in the cellular response to replication stress ensuring accurate DNA replication and repair. It does not function as a single entity but acts as part of the ATR activation complex which is involved in sensing and signaling DNA damage. By facilitating ATR activation ETAA1 helps coordinate cell cycle checkpoints and repair processes maintaining genomic integrity under stress conditions.

Pathways

ETAA1 integrates into the DNA damage response pathway and cellular cycle regulation. It operates closely with ATR within the DDR (DNA Damage Response) pathway sharing functional interactions with proteins like TopBP1 and Rad17 which also contribute to ATR activation. Together these proteins ensure the monitoring and repairing of DNA lesions therefore preventing the accumulation of mutations.

ETAA1 associates with cancer and developmental disorders linked to genomic instability. Its dysfunction or altered expression can disrupt the DNA damage response potentially leading to the accumulation of mutations seen in different cancers. The interaction of ETAA1 with ATR emphasizes this relationship as both proteins become pivotal in responding to DNA damage and maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity (PubMed : 27601467, PubMed : 27723717, PubMed : 27723720). Recruited to stalled replication forks via interaction with the RPA complex and directly stimulates ATR kinase activity independently of TOPBP1 (PubMed : 27723717, PubMed : 27723720, PubMed : 30139873). Probably only regulates a subset of ATR targets (PubMed : 27723717, PubMed : 27723720).
See full target information ETAA1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7799 PubMed40921755

2025

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Kyohei Matsuhashi,Kei K Ito,Kaho Nagai,Akira Sanada,Koki Watanabe,Kasuga Takumi,Atsushi Toyoda,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Grant S Stewart,Shoji Hata,Daiju Kitagawa

The Journal of biological chemistry 291:21956-21962 PubMed27601467

2016

Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks.

Applications

WB

Species

Human

Sumin Feng,Yichao Zhao,Yixi Xu,Shaokai Ning,Wei Huo,Mei Hou,Ge Gao,Jianguo Ji,Rong Guo,Dongyi Xu
View all publications
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