AB243944

Anti-ETAA1 antibody [EPR14052(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ETAA1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

ETAA16, ETAA1, Ewing's tumor-associated antigen 1, Ewing's tumor-associated antigen 16

1 Images

Supplier Data

Western blot - Anti-ETAA1 antibody [EPR14052(2)] - BSA and Azide free (AB243944)

Blocking/dilution buffer : 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197017).

All lanes:

Western blot - Anti-ETAA1 antibody [EPR14052(2)] (<a href='/en-us/products/primary-antibodies/etaa1-antibody-epr140522-ab197017'>ab197017</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 103 kDa

Observed band size: 90 kDa

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Unconjugated

Anti-ETAA1 antibody [EPR14052(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14052(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab243944 is the carrier-free version of ab197017.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETAA1 also known as Ewing tumor-associated antigen 1 is a protein recognized for its role in DNA replication stress response. It is approximately 92 kDa in mass and ubiquitously expressed in various human tissues. ETAA1 functions at the mechanical level by activating the ATR (ataxia telangiectasia and Rad3-related) kinase an enzyme critical for maintaining genomic stability. This protein contains ATR activators that allow the phosphorylation of various substrates participating in DNA damage repair.

Biological function summary

ETAA1 plays a significant role in the cellular response to replication stress ensuring accurate DNA replication and repair. It does not function as a single entity but acts as part of the ATR activation complex which is involved in sensing and signaling DNA damage. By facilitating ATR activation ETAA1 helps coordinate cell cycle checkpoints and repair processes maintaining genomic integrity under stress conditions.

Pathways

ETAA1 integrates into the DNA damage response pathway and cellular cycle regulation. It operates closely with ATR within the DDR (DNA Damage Response) pathway sharing functional interactions with proteins like TopBP1 and Rad17 which also contribute to ATR activation. Together these proteins ensure the monitoring and repairing of DNA lesions therefore preventing the accumulation of mutations.

ETAA1 associates with cancer and developmental disorders linked to genomic instability. Its dysfunction or altered expression can disrupt the DNA damage response potentially leading to the accumulation of mutations seen in different cancers. The interaction of ETAA1 with ATR emphasizes this relationship as both proteins become pivotal in responding to DNA damage and maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity (PubMed : 27601467, PubMed : 27723717, PubMed : 27723720). Recruited to stalled replication forks via interaction with the RPA complex and directly stimulates ATR kinase activity independently of TOPBP1 (PubMed : 27723717, PubMed : 27723720, PubMed : 30139873). Probably only regulates a subset of ATR targets (PubMed : 27723717, PubMed : 27723720).

See full target information ETAA1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETAA1 antibody [EPR14052(2)] - BSA and Azide free

Western blot - Anti-ETAA1 antibody [EPR14052(2)] - BSA and Azide free (AB243944)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise