AB153722

Anti-ETFA antibody

Name: Anti-ETFA antibody (ab153722) | Abcam
Brand: Abcam Limited
SKU: AB153722
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal ETFA antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat, Zebrafish, Recombinant fragment samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETFA aa 50 to C-terminus.

View Alternative Names

Alpha-ETF, ETFA

12 Images

Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody (AB153722)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody (AB153722)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling ETFA with ab153722 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Paraffin-embedded mouse kidney tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Paraffin-embedded mouse cervix tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Paraffin-embedded mouse urinary bladder tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Paraffin-embedded mouse brain stem tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ETFA antibody (AB153722)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-ETFA antibody (ab153722) at 1/1000 dilution

Lane 1:

Wild-type ETFA 293T cell extracts at 30 µg

Lane 2:

Knockout ETFA 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-ETFA antibody (AB153722)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-ETFA antibody (ab153722) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-ETFA antibody (AB153722)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ETFA antibody (ab153722) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Unknown

Western blot - Anti-ETFA antibody (AB153722)

10 % SDS-PAGE

All lanes:

Western blot - Anti-ETFA antibody (ab153722)

All lanes:

C8D30 whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Supplier Data

Immunoprecipitation - Anti-ETFA antibody (AB153722)

ETFA was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 μg ab153722. Western blot was performed from the immunoprecipitate using ab153722.

Lane 1 : IgG instead of ab153722 in HepG2 whole cell lysate.

Lane 2 : ab153722 IP in HepG2 whole cell lysate.

All lanes:

Immunoprecipitation - Anti-ETFA antibody (ab153722)

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-ETFA antibody (AB153722)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ETFA antibody (ab153722) at 1/500 dilution

Lane 1:

Zebrafish muscle tissue extracts at 30 µg

Lane 2:

Whole zebrafish tissue extracts at 30 µg

Predicted band size: 35 kDa

false

Unknown

Western blot - Anti-ETFA antibody (AB153722)

10 % SDS-PAGE

All lanes:

Western blot - Anti-ETFA antibody (ab153722) at 1/1000 dilution

All lanes:

PC12 whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB, IP, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ETFA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13804

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETFA or Electron-Transfer-Flavoprotein alpha subunit is an essential part of the mitochondrial respiratory chain. This protein which has a molecular mass of approximately 34 kDa functions in the transfer of electrons from acyl-CoA dehydrogenases to the main respiratory chain for energy production. ETFA is commonly expressed in tissues with high-energy demands such as the liver heart and skeletal muscle. The protein forms a heterodimeric complex with its counterpart ETFB providing a critical function in electron transfer during fatty acid oxidation.

Biological function summary

ETFA operates as an important part of the electron transfer process within the mitochondria. It acts as one-half of the heterodimeric electron-transfer flavoprotein complex teaming with ETFB. This complex facilitates electron transfer from a range of acyl-CoA dehydrogenases to ETF dehydrogenase which then continues the process of electron transfer to coenzyme Q in the respiratory chain. This action is key to the breakdown of fats enabling energy extraction and processing.

Pathways

ETFA has important roles in fatty acid beta-oxidation and amino acid catabolism. It engages in these pathways by transferring electrons as mentioned interfacing with other proteins like ETF dehydrogenase. This positioning within the mitochondrial matrix enables ETFA to assist in converting fat and protein substrates into energy which the cell can use. Its molecular interactions highlight its integral position in maintaining energy homeostasis.

Problems with ETFA lead to glutaric acidemia type 2 a metabolic disorder that impairs the body's ability to oxidize fatty acids and some amino acids. Deficiencies in ETFA function disrupt the electron transport to the respiratory chain causing an accumulation of intermediary metabolites. These disruptions can relate to or involve other mitochondrial components and proteins like ETFB or ETFDH. Correct diagnosis and understanding of ETFA’s role in such conditions are instrumental for targeted therapeutic approaches.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed : 10356313, PubMed : 15159392, PubMed : 15975918, PubMed : 27499296, PubMed : 9334218). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed : 9334218). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed : 12815589, PubMed : 1430199, PubMed : 1882842).

See full target information ETFA

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:1362 PubMed39779759

2025

Tryptophan metabolism-related gene CYP1B1 serves as a shared biomarker for both Parkinson's disease and insomnia.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Li,Wen-Kai Yu,Jing-Hao Wu,Wen-Jun He,Yu-Nan Cheng,Kai Gao,Yi-Han Wei,Yu-Sheng Li

Journal of cell communication and signaling 18:e12038 PubMed38946717

2024

Enoyl coenzyme a hydratase 1 attenuates aortic valve calcification by suppressing Runx2 via Wnt5a/Ca pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Caijun Rao,Baoqing Liu,Haojie Qin,Zhipeng Du

Journal of cellular biochemistry 120:7458-7473 PubMed30387180

2018

Repression of let-7b-5p prevents the development of multifidus muscle dysfunction by promoting vitamin D accumulation via upregulation of electron transfer flavoprotein alpha subunit in a rat model of multifidus muscle injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Ming Yu,Xue-Dong Yao,Rong-Mou Zhang,Hua-Feng Zhuang,Pei-Wen Wang,Yi-Zhong Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETFA antibody

Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody (AB153722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Immunoprecipitation - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Western blot - Anti-ETFA antibody (AB153722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise