Goat Polyclonal ETFB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ETFB aa 150-200.
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- Proteomics. Clinical applications 10:1205-12172016Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly L Stauch,Lance M Villeneuve,Phillip R Purnell,Brendan M Ottemann,Katy Emanuel,Howard S FoxPubMed 27568932
- Molecular medicine reports 14:681-82016Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ETFB aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P38117
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:15159392, PubMed:15975918, PubMed:25416781). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed:15159392, PubMed:15975918, PubMed:8962055).
Alternative names
FP585, ETFB, Electron transfer flavoprotein subunit beta, Beta-ETF
Recommended products
Goat Polyclonal ETFB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ETFB aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ETFB aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P38117
- Purification technique
- Affinity purification Immunogen
- Specificity
ab104944 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
- Concentration
- Purification notes
ab104944 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ETFB also known as electron transfer flavoprotein subunit beta is a protein that plays an important role in transferring electrons in cellular energy metabolism. This protein has a molecular mass of approximately 27 kDa and is mainly expressed in mitochondria-rich organs such as the liver heart and muscle tissues. This protein pairs with the alpha subunit (ETFA) to form the heterodimeric structure of the electron transfer flavoprotein (ETF) essential for its function. ETFB's presence is significant in tissues with high metabolic demand aligning with its involvement in energy production processes.
- Biological function summary
The ETFB protein partners with ETFA to form the complete ETF complex which serves as an important electron transporter in the mitochondria. It accepts electrons from various flavoprotein dehydrogenases and transfers them to the mitochondrial respiratory chain. This process is important for beta-oxidation of fatty acids and catabolism of some amino acids. ETFB ensures proper energy production and cellular respiration and its deficiency can lead to severe metabolic disruptions.
- Pathways
Several important biological pathways involve ETFB most notably the fatty acid oxidation pathway and the branched-chain amino acid catabolism. It interacts closely with proteins such as the ACADs (acyl-CoA dehydrogenases) which directly send electrons to ETF in the mitochondria. This interaction allows ETFB to maintain efficient energy conversion processes directly linking its function to metabolic activity and energy homeostasis.
- Associated diseases and disorders
ETFB's dysfunction associates with metabolic disorders like glutaric aciduria type II (GA II) and multiple acyl-CoA dehydrogenase deficiency (MADD). These conditions stem from defects in the electron transport function of the ETF complex leading to energy production impairment. Patients with these disorders may have symptoms ranging from mild to severe metabolic crises. ETFB's disturbance in this context often involves ETFA as both proteins form the ETF complex important for normal metabolic function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ETFB antibody (ab104944)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-ETFB antibody (ab104944) at 0.1 µg/mL
All lanes: Human Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 27 kDa
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