AB104944

Anti-ETFB antibody

Name: Anti-ETFB antibody (ab104944) | Abcam
Brand: Abcam Limited
SKU: AB104944
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal ETFB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ETFB aa 150-200.

View Alternative Names

FP585, ETFB, Electron transfer flavoprotein subunit beta, Beta-ETF

1 Images

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Western blot - Anti-ETFB antibody (AB104944)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ETFB antibody (ab104944) at 0.1 µg/mL

All lanes:

Human Liver lysate (in RIPA buffer) at 35 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ETFB aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P38117

Specificity

ab104944 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab104944 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETFB also known as electron transfer flavoprotein subunit beta is a protein that plays an important role in transferring electrons in cellular energy metabolism. This protein has a molecular mass of approximately 27 kDa and is mainly expressed in mitochondria-rich organs such as the liver heart and muscle tissues. This protein pairs with the alpha subunit (ETFA) to form the heterodimeric structure of the electron transfer flavoprotein (ETF) essential for its function. ETFB's presence is significant in tissues with high metabolic demand aligning with its involvement in energy production processes.

Biological function summary

The ETFB protein partners with ETFA to form the complete ETF complex which serves as an important electron transporter in the mitochondria. It accepts electrons from various flavoprotein dehydrogenases and transfers them to the mitochondrial respiratory chain. This process is important for beta-oxidation of fatty acids and catabolism of some amino acids. ETFB ensures proper energy production and cellular respiration and its deficiency can lead to severe metabolic disruptions.

Pathways

Several important biological pathways involve ETFB most notably the fatty acid oxidation pathway and the branched-chain amino acid catabolism. It interacts closely with proteins such as the ACADs (acyl-CoA dehydrogenases) which directly send electrons to ETF in the mitochondria. This interaction allows ETFB to maintain efficient energy conversion processes directly linking its function to metabolic activity and energy homeostasis.

ETFB's dysfunction associates with metabolic disorders like glutaric aciduria type II (GA II) and multiple acyl-CoA dehydrogenase deficiency (MADD). These conditions stem from defects in the electron transport function of the ETF complex leading to energy production impairment. Patients with these disorders may have symptoms ranging from mild to severe metabolic crises. ETFB's disturbance in this context often involves ETFA as both proteins form the ETF complex important for normal metabolic function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed : 15159392, PubMed : 15975918, PubMed : 25416781). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed : 12815589, PubMed : 7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed : 15159392, PubMed : 15975918, PubMed : 8962055).

See full target information ETFB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proteomics. Clinical applications 10:1205-1217 PubMed27568932

2016

Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Stauch,Lance M Villeneuve,Phillip R Purnell,Brendan M Ottemann,Katy Emanuel,Howard S Fox

Molecular medicine reports 14:681-8 PubMed27222353

2016

Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction.

Applications

Species

Rat

Qi Zhao,Yansheng Xue,Yi Yang,Zhibin Niu,Changlin Wang,Ying Hou,Hui Chen

Free radical biology & medicine 73:21-33 PubMed24751525

2014

Frataxin deficiency in neonatal rat ventricular myocytes targets mitochondria and lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Èlia Obis,Verónica Irazusta,Daniel Sanchís,Joaquim Ros,Jordi Tamarit

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ETFB antibody

Western blot - Anti-ETFB antibody (AB104944)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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