AB240593

Anti-ETFB antibody

Name: Anti-ETFB antibody (ab240593) | Abcam
Brand: Abcam Limited
SKU: AB240593
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ETFB antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ETFB aa 200 to C-terminus.

View Alternative Names

FP585, ETFB, Electron transfer flavoprotein subunit beta, Beta-ETF

2 Images

Supplier Data

Immunoprecipitation - Anti-ETFB antibody (AB240593)

ETFB was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer with ab240593 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab240593 at 0.4 μg/mL.

Lane 1 : ab240593 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG (-).

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ETFB antibody (ab240593)

Predicted band size: 27 kDa

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Supplier Data

Western blot - Anti-ETFB antibody (AB240593)

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-ETFB antibody (ab240593) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ETFB aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P38117

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETFB also known as electron transfer flavoprotein subunit beta is a protein that plays an important role in transferring electrons in cellular energy metabolism. This protein has a molecular mass of approximately 27 kDa and is mainly expressed in mitochondria-rich organs such as the liver heart and muscle tissues. This protein pairs with the alpha subunit (ETFA) to form the heterodimeric structure of the electron transfer flavoprotein (ETF) essential for its function. ETFB's presence is significant in tissues with high metabolic demand aligning with its involvement in energy production processes.

Biological function summary

The ETFB protein partners with ETFA to form the complete ETF complex which serves as an important electron transporter in the mitochondria. It accepts electrons from various flavoprotein dehydrogenases and transfers them to the mitochondrial respiratory chain. This process is important for beta-oxidation of fatty acids and catabolism of some amino acids. ETFB ensures proper energy production and cellular respiration and its deficiency can lead to severe metabolic disruptions.

Pathways

Several important biological pathways involve ETFB most notably the fatty acid oxidation pathway and the branched-chain amino acid catabolism. It interacts closely with proteins such as the ACADs (acyl-CoA dehydrogenases) which directly send electrons to ETF in the mitochondria. This interaction allows ETFB to maintain efficient energy conversion processes directly linking its function to metabolic activity and energy homeostasis.

ETFB's dysfunction associates with metabolic disorders like glutaric aciduria type II (GA II) and multiple acyl-CoA dehydrogenase deficiency (MADD). These conditions stem from defects in the electron transport function of the ETF complex leading to energy production impairment. Patients with these disorders may have symptoms ranging from mild to severe metabolic crises. ETFB's disturbance in this context often involves ETFA as both proteins form the ETF complex important for normal metabolic function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed : 15159392, PubMed : 15975918, PubMed : 25416781). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed : 12815589, PubMed : 7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed : 15159392, PubMed : 15975918, PubMed : 8962055).

See full target information ETFB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 51: PubMed38391012

2024

Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Junyi Wu,Huiqin Zhu,Xiaoqin Shi,Jun Liu,Yang Li,Min Wang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:4602-4618 PubMed32030805

2020

Mitochondrial adaptations to exercise do not require Bcl2-mediated autophagy but occur with BNIP3/Parkin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Ehrlicher,Harrison D Stierwalt,Benjamin F Miller,Sean A Newsom,Matthew M Robinson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETFB antibody

Immunoprecipitation - Anti-ETFB antibody (AB240593)

Western blot - Anti-ETFB antibody (AB240593)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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