Anti-ETFDH antibody [3H2BG1]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3H2BG1] (AB126576)

IHC image of ETFDH staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126576, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Unknown

Western blot - Anti-ETFDH antibody [3H2BG1] (AB126576)

The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the goat anti-Mouse secondary antibody.

All lanes:

Western blot - Anti-ETFDH antibody [3H2BG1] (ab126576) at 1 µg/mL

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human cell lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Lane 5:

Mouse liver lysate at 20 µg

Lane 6:

Bovine heart mitochondria at 10 µg

Secondary

All lanes:

GAM-HRP at 1/3000 dilution

Predicted band size: 68 kDa

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