AB224663

Anti-ETFDH antibody - C-terminal

Name: Anti-ETFDH antibody - C-terminal (ab224663) | Abcam
Brand: Abcam Limited
SKU: AB224663
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal ETFDH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus.

View Alternative Names

ETF-QO, ETF-ubiquinone oxidoreductase, Electron-transferring-flavoprotein dehydrogenase, ETF dehydrogenase, ETFDH

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663)

Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663)

Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (AB224663)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (AB224663)

PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-ETFDH antibody - C-terminal (AB224663)

All lanes:

Western blot - Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution

Lane 1:

Mouse liver tissue lysate

Lane 2:

Mouse kidney tissue lysate

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16134

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETFDH also known as Electron Transfer Flavoprotein Dehydrogenase plays an important role in transferring electrons within the mitochondrial respiratory chain. With a molecular mass of approximately 65 kDa it is predominantly expressed in tissues with high energy demands such as muscle and liver. ETFDH operates as a component of the electron transport chain facilitating electron transfer from the electron transfer flavoprotein (ETF) to the ubiquinone pool.

Biological function summary

The action of ETFDH assists in fatty acid and amino acid metabolism. As part of the larger electron transport chain complex ETFDH partners with both ETF-alpha and ETF-beta subunits to facilitate electron transfer. This process is key in the oxidation of fatty acids particularly in the beta-oxidation pathway. The functional activity of ETFDH helps convert energy stored in macronutrients into adenosine triphosphate (ATP) a necessary energy currency in cells.

Pathways

ETFDH has a critical role in both the beta-oxidation of fatty acids and the catabolism of certain amino acids. Within these pathways it acts as an intersection between the mitochondria and cellular energy production. ETFDH operates with other proteins such as ACAD9 and ACADVL to ensure efficient energy conversion and maintain cellular homeostasis. These pathways highlight ETFDH's involvement in mitochondrial function and overall energy metabolism.

Mutations in the ETFDH gene are linked to conditions like multiple acyl-CoA dehydrogenase deficiency (MADD) and glutaric acidemia type II. These metabolic disorders arise from deficiencies in energy metabolism often leading to severe clinical symptoms. ETFDH's interaction with proteins like ACAD9 can influence the severity and onset of these conditions emphasizing its medical significance in metabolic health. Understanding ETFDH's operation provides insight into potential therapeutic targets for related metabolic diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts electrons from ETF and reduces ubiquinone.

See full target information ETFDH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 7:fcaf182 PubMed40462859

2025

Intra-amniotic infection with causes serovar-dependent white matter damage in preterm fetal sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Dima Abdu,Graeme R Polglase,Sharmony B Kelly,Sinead Murphy,Ilias Nitsos,Claudia A Nold-Petry,Suhas G Kallapur,Alan H Jobe,John P Newnham,Timothy J Moss,Robert Galinsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETFDH antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663)

Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (AB224663)

Western blot - Anti-ETFDH antibody - C-terminal (AB224663)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise