Rabbit Polyclonal ETFDH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus.
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Expected | Tested | Tested |
Mouse | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Accepts electrons from ETF and reduces ubiquinone.
ETF-QO, ETF-ubiquinone oxidoreductase, Electron-transferring-flavoprotein dehydrogenase, ETF dehydrogenase, ETFDH
Rabbit Polyclonal ETFDH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus.
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ETFDH also known as Electron Transfer Flavoprotein Dehydrogenase plays an important role in transferring electrons within the mitochondrial respiratory chain. With a molecular mass of approximately 65 kDa it is predominantly expressed in tissues with high energy demands such as muscle and liver. ETFDH operates as a component of the electron transport chain facilitating electron transfer from the electron transfer flavoprotein (ETF) to the ubiquinone pool.
The action of ETFDH assists in fatty acid and amino acid metabolism. As part of the larger electron transport chain complex ETFDH partners with both ETF-alpha and ETF-beta subunits to facilitate electron transfer. This process is key in the oxidation of fatty acids particularly in the beta-oxidation pathway. The functional activity of ETFDH helps convert energy stored in macronutrients into adenosine triphosphate (ATP) a necessary energy currency in cells.
ETFDH has a critical role in both the beta-oxidation of fatty acids and the catabolism of certain amino acids. Within these pathways it acts as an intersection between the mitochondria and cellular energy production. ETFDH operates with other proteins such as ACAD9 and ACADVL to ensure efficient energy conversion and maintain cellular homeostasis. These pathways highlight ETFDH's involvement in mitochondrial function and overall energy metabolism.
Mutations in the ETFDH gene are linked to conditions like multiple acyl-CoA dehydrogenase deficiency (MADD) and glutaric acidemia type II. These metabolic disorders arise from deficiencies in energy metabolism often leading to severe clinical symptoms. ETFDH's interaction with proteins like ACAD9 can influence the severity and onset of these conditions emphasizing its medical significance in metabolic health. Understanding ETFDH's operation provides insight into potential therapeutic targets for related metabolic diseases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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