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Rabbit Polyclonal ETFDH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus.


Images

Western blot - Anti-ETFDH antibody - C-terminal (AB224663), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (AB224663), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (AB224663), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16134

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Accepts electrons from ETF and reduces ubiquinone.

Alternative names

Recommended products

Rabbit Polyclonal ETFDH antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ETFDH aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16134
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ETFDH also known as Electron Transfer Flavoprotein Dehydrogenase plays an important role in transferring electrons within the mitochondrial respiratory chain. With a molecular mass of approximately 65 kDa it is predominantly expressed in tissues with high energy demands such as muscle and liver. ETFDH operates as a component of the electron transport chain facilitating electron transfer from the electron transfer flavoprotein (ETF) to the ubiquinone pool.

Biological function summary

The action of ETFDH assists in fatty acid and amino acid metabolism. As part of the larger electron transport chain complex ETFDH partners with both ETF-alpha and ETF-beta subunits to facilitate electron transfer. This process is key in the oxidation of fatty acids particularly in the beta-oxidation pathway. The functional activity of ETFDH helps convert energy stored in macronutrients into adenosine triphosphate (ATP) a necessary energy currency in cells.

Pathways

ETFDH has a critical role in both the beta-oxidation of fatty acids and the catabolism of certain amino acids. Within these pathways it acts as an intersection between the mitochondria and cellular energy production. ETFDH operates with other proteins such as ACAD9 and ACADVL to ensure efficient energy conversion and maintain cellular homeostasis. These pathways highlight ETFDH's involvement in mitochondrial function and overall energy metabolism.

Associated diseases and disorders

Mutations in the ETFDH gene are linked to conditions like multiple acyl-CoA dehydrogenase deficiency (MADD) and glutaric acidemia type II. These metabolic disorders arise from deficiencies in energy metabolism often leading to severe clinical symptoms. ETFDH's interaction with proteins like ACAD9 can influence the severity and onset of these conditions emphasizing its medical significance in metabolic health. Understanding ETFDH's operation provides insight into potential therapeutic targets for related metabolic diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-ETFDH antibody - C-terminal (ab224663), expandable thumbnail

    Western blot - Anti-ETFDH antibody - C-terminal (ab224663)

    All lanes: Western blot - Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution

    Lane 1: Mouse liver tissue lysate

    Lane 2: Mouse kidney tissue lysate

    Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

    Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

    Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)

    PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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