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AB251791

Anti-ETHE1 antibody

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(1 Publication)

Rabbit Polyclonal ETHE1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ETHE1 aa 150-250.

View Alternative Names

HSCO, ETHE1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, Sulfur dioxygenase ETHE1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)

Formalin-fixed, paraffin-embedded human liver tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)

Formalin-fixed, paraffin-embedded human kidney tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ETHE1 antibody (AB251791)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ETHE1 antibody (AB251791)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ETHE1 (green) using ab251791 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETHE1 antibody (AB251791)

Formalin-fixed, paraffin-embedded human colon tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-ETHE1 antibody (AB251791)
  • WB

Unknown

Western blot - Anti-ETHE1 antibody (AB251791)

All lanes:

Western blot - Anti-ETHE1 antibody (ab251791) at 0.4 µg/mL

Lane 1:

RT-4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

Human U-251-MG sp whole cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 28 kDa

false

Western blot - Anti-ETHE1 antibody (AB251791)
  • WB

Unknown

Western blot - Anti-ETHE1 antibody (AB251791)

All lanes:

Western blot - Anti-ETHE1 antibody (ab251791) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II whole cell lysate

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ETHE1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O95571

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETHE1 also known as persulfide dioxygenase plays an important role in the breakdown of sulfur-containing compounds. It catalyzes the conversion of persulfides to sulfite in the mitochondrial matrix. The protein has an approximate mass of 26 kDa and is strongly expressed in tissues with high metabolic activity such as the liver kidney and muscle. It plays an important role in maintaining cellular function and energy balance by efficiently processing by-products of cysteine metabolism.
Biological function summary

ETHE1 assists in detoxifying potentially harmful sulfur compounds. It forms a part of a complex with sulfide:quinone oxidoreductase (SQR) and thiosulfate sulfurtransferase (TST) to facilitate efficient sulfur metabolism. The importance of ETHE1 lies in its ability to prevent the accumulation of toxic sulfide levels which can impair cellular respiration and energy production. The protein's role as a persulfide dioxygenase places it in a unique position as one of the body's defenses against sulfide toxicity.

Pathways

ETHE1 has significant relevance in the sulfide oxidation pathway and cellular respiration processes. Particularly it interacts with SQR in the mitochondrial matrix to convert hydrogen sulfide to thiosulfate and sulfite which then enter the citric acid cycle. This critical pathway is associated with energy production and efficient cellular function. The cooperation between ETHE1 and SQR ensures that toxic sulfide levels do not rise protecting mitochondrial integrity.

Defects in ETHE1 result in ethylmalonic encephalopathy a severe metabolic disorder. The lack of functional ETHE1 leads to the accumulation of toxic levels of hydrogen sulfide which may disrupt mitochondrial function and energy production. This disorder highlights the interconnectedness of ETHE1 with SQR as impairments in this protein complex can exacerbate the pathological condition. Understanding the role of ETHE1 in this disease helps target therapies aimed at reducing sulfide toxicity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus (PubMed : 12398897).
See full target information ETHE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:6027-6036 PubMed32606808

2020

Upregulated lncRNA CASC9 Contributes to Progression of Non-Small Cell Lung Cancer Through Inhibition of miR-335-3p and Activation S100A14 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weigang Zhao,Tangbing Chen,Yonghong Zhao
View all publications
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Product promise

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