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AB124282

Anti-ETS1 antibody

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(8 Publications)

Rabbit Polyclonal ETS1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ETS1 aa 150-250.

View Alternative Names

EWSR2, ETS1, Protein C-ets-1, p54

2 Images
Immunoprecipitation - Anti-ETS1 antibody (AB124282)
  • IP

Unknown

Immunoprecipitation - Anti-ETS1 antibody (AB124282)

ab124282 at 1 µg/ml staining ETS1 in Jurkat whole cell lysate immunoprecipitated using ab124282 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : control IgG.
Predicted band size : 50 kDa. Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-ETS1 antibody (ab124282)

Predicted band size: 50 kDa

false

Western blot - Anti-ETS1 antibody (AB124282)
  • WB

Unknown

Western blot - Anti-ETS1 antibody (AB124282)

All lanes:

Western blot - Anti-ETS1 antibody (ab124282) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ETS1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P14921

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab124282 was affinity purified using an epitope specific to ETS1 immobilized on solid support .
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETS1 also known as E26 Transformation-Specific 1 is a transcription factor protein weighing approximately 51-53 kDa. It belongs to the ETS family of transcription factors and can be referred to by other names such as ETS-1 ETS 1 or Ets-1. This protein shows expression in numerous tissues including the thymus spleen and brain. Importantly ETS1 binds to specific DNA sequences to regulate the transcription of various target genes.
Biological function summary

The function of this protein involves the regulation of gene expression linked to cell development differentiation and apoptosis. ETS1 does not form stable complexes with other proteins in its resting form but it can interact dynamically with co-factors during activation. Its activity plays a role in immune response modulation and vascular development by controlling downstream target genes connected to these processes.

Pathways

The involvement of this protein spans key signal transduction pathways including the MAPK and PI3K/AKT pathways. In these pathways ETS1 regulates gene expression influenced by external stimuli such as growth factors. It interacts with other pathway-related proteins like ERK1/2 and AKT initiating cellular responses that contribute to cell proliferation and survival mechanisms.

Abnormalities in the function or expression of this protein have been linked to certain cancers such as leukemia and breast cancer. The protein interacts with other oncogenic proteins including BRISA and Palikuqi leading to progression of these malignancies. Additionally involvement in autoimmune disorders like lupus has been observed where deregulation of ETS1 can affect immune cell behavior and promote hyperactivation of immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor (PubMed : 10698492, PubMed : 11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed : 20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed : 20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed : 15247905, PubMed : 15592518).. Isoform Ets-1 p27. Acts as a dominant-negative for isoform c-ETS-1A.
See full target information ETS1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e109803 PubMed36917143

2023

Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1-Runx1 complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Duy Pham,Daniel J Silberger,Kim N Nguyen,Min Gao,Casey T Weaver,Robin D Hatton

Open medicine (Warsaw, Poland) 16:1564-1582 PubMed34722892

2021

Superenhancer-transcription factor regulatory network in malignant tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liang,Linlin Li,Tian Xin,Binru Li,Dalin Zhang

Journal of leukocyte biology 111:19-32 PubMed34448502

2021

Adipose-derived stem cell-derived extracellular vesicles inhibit neuroblastoma growth by regulating GABBR1 activity through LINC00622-mediated transcription factor AR.

Applications

Unspecified application

Species

Unspecified reactive species

Mengguo Guo,Dongpeng Li,Yawen Feng,Mu Li,Bo Yang

OncoTargets and therapy 13:921-930 PubMed32099396

2020

Long Noncoding RNA SNHG6 Functions as an Oncogene in Non-Small Cell Lung Cancer via Modulating ETS1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Geng,Shixiong Li,Meilin Xu

Cell reports 26:1286-1302.e8 PubMed30699355

2019

Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Piotr K Zadora,Cindrilla Chumduri,Koshi Imami,Hilmar Berger,Yang Mi,Matthias Selbach,Thomas F Meyer,Rajendra Kumar Gurumurthy

Molecular cancer therapeutics 16:2656-2667 PubMed28878027

2017

Death by HDAC Inhibition in Synovial Sarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aimée N Laporte,Neal M Poulin,Jared J Barrott,Xiu Qing Wang,Alireza Lorzadeh,Ryan Vander Werff,Kevin B Jones,T Michael Underhill,Torsten O Nielsen

PloS one 12:e0169407 PubMed28056055

2017

HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma.

Applications

WB

Species

Human

Aimée N Laporte,Jared J Barrott,Ren Jie Yao,Neal M Poulin,Bertha A Brodin,Kevin B Jones,T Michael Underhill,Torsten O Nielsen

Transcription 3:323-35 PubMed23117818

2012

Ets1 and Elk1 transcription factors regulate cancerous inhibitor of protein phosphatase 2A expression in cervical and endometrial carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rajash Pallai,Aishwarya Bhaskar,Valerie Sodi,Lyndi M Rice
View all publications
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