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AB225868

Anti-ETS1 antibody

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(7 Publications)

Rabbit Polyclonal ETS1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ETS1 aa 350 to C-terminus.

View Alternative Names

EWSR2, ETS1, Protein C-ets-1, p54

2 Images
Immunoprecipitation - Anti-ETS1 antibody (AB225868)
  • IP

Supplier Data

Immunoprecipitation - Anti-ETS1 antibody (AB225868)

ETS1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225868 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225868 at 1 μg/ml.

Lane 1 : ab225868 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ETS1 antibody (ab225868)

Predicted band size: 50 kDa

false

Western blot - Anti-ETS1 antibody (AB225868)
  • WB

Supplier Data

Western blot - Anti-ETS1 antibody (AB225868)

All lanes:

Western blot - Anti-ETS1 antibody (ab225868) at 0.1 µg/mL

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ETS1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P14921

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETS1 also known as E26 Transformation-Specific 1 is a transcription factor protein weighing approximately 51-53 kDa. It belongs to the ETS family of transcription factors and can be referred to by other names such as ETS-1 ETS 1 or Ets-1. This protein shows expression in numerous tissues including the thymus spleen and brain. Importantly ETS1 binds to specific DNA sequences to regulate the transcription of various target genes.
Biological function summary

The function of this protein involves the regulation of gene expression linked to cell development differentiation and apoptosis. ETS1 does not form stable complexes with other proteins in its resting form but it can interact dynamically with co-factors during activation. Its activity plays a role in immune response modulation and vascular development by controlling downstream target genes connected to these processes.

Pathways

The involvement of this protein spans key signal transduction pathways including the MAPK and PI3K/AKT pathways. In these pathways ETS1 regulates gene expression influenced by external stimuli such as growth factors. It interacts with other pathway-related proteins like ERK1/2 and AKT initiating cellular responses that contribute to cell proliferation and survival mechanisms.

Abnormalities in the function or expression of this protein have been linked to certain cancers such as leukemia and breast cancer. The protein interacts with other oncogenic proteins including BRISA and Palikuqi leading to progression of these malignancies. Additionally involvement in autoimmune disorders like lupus has been observed where deregulation of ETS1 can affect immune cell behavior and promote hyperactivation of immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor (PubMed : 10698492, PubMed : 11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed : 20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed : 20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed : 15247905, PubMed : 15592518).. Isoform Ets-1 p27. Acts as a dominant-negative for isoform c-ETS-1A.
See full target information ETS1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:426 PubMed40456737

2025

FDA-approved phensuximide inhibits RIPK1-dependent immunogenic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Byeong-Ju Kim,Sun Mi Hong,Hyun-Jin Noh,Jihye Kim,Su-Yeon Seon,Jeong-Eun Lee,Da-Hye Jeong,Ju-Mi Park,Sejeong Park,Sanghoon Lee,Jaewoo Kang,Dakeun Lee,Michael J Morgan,You-Sun Kim

Cell reports. Medicine 4:100953 PubMed36809766

2023

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Li Cao,Wen-Jun Gu,Lei Li,Zhong-Zhong Chen,Jie Xiang,Ze-Yu Zhou,Bo Xu,Wei-Dong Zang,Xiang-Yu Zhou,Jing Cao,Kun Sun,Jian-Yuan Zhao

Bioengineered 13:12927-12940 PubMed35611792

2022

Knocking down ETS Proto-oncogene 1 (ETS1) alleviates the pyroptosis of renal tubular epithelial cells in patients with acute kidney injury by regulating the NLR family pyrin domain containing 3 (NLRP3) transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxia Juan,Ye Zhu,Yan Chen,Yan Mao,Yan Zhou,Weiwei Zhu,Xufang Wang,Qian Wang

Oncology reports 46: PubMed34278501

2021

Mechanism of tumor‑derived extracellular vesicles in regulating renal cell carcinoma progression by the delivery of MALAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Chengluo Jin,Linmei Shi,Kunlun Li,Wei Liu,Yu Qiu,Yakun Zhao,Bai Zhao,Zhexun Li,Yifei Li,Qingguo Zhu

Nature 585:426-432 PubMed32908310

2020

Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Brisa Palikuqi,Duc-Huy T Nguyen,Ge Li,Ryan Schreiner,Alessandro F Pellegata,Ying Liu,David Redmond,Fuqiang Geng,Yang Lin,Jesus M Gómez-Salinero,Masataka Yokoyama,Paul Zumbo,Tuo Zhang,Balvir Kunar,Mavee Witherspoon,Teng Han,Alfonso M Tedeschi,Federico Scottoni,Steven M Lipkin,Lukas Dow,Olivier Elemento,Jenny Z Xiang,Koji Shido,Jason R Spence,Qiao J Zhou,Robert E Schwartz,Paolo De Coppi,Sina Y Rabbany,Shahin Rafii

Journal of experimental & clinical cancer research : CR 38:486 PubMed31823805

2019

Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyang Xu,Guifang Zhao,Yu Zhang,Hong Jiang,Weiyao Wang,Donghai Zhao,Hongquan Yu,Ling Qi

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:7037-7048 PubMed30870006

2019

Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Lei,Hui-Qing Li,Zhi-Huai Mo,Ke-Jia Liu,Ling-Juan Zhu,Chun-Yi Li,Wen-Li Chen,Lei Zhang
View all publications
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