Skip to main content

Rabbit Recombinant Monoclonal ETS1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ETS1 antibody [EPR21909] (AB220361), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361), expandable thumbnail
  • Western blot - Anti-ETS1 antibody [EPR21909] (AB220361), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518). Isoform Ets-1 p27. Acts as a dominant-negative for isoform c-ETS-1A.

Alternative names

EWSR2, ETS1, Protein C-ets-1, p54

Recommended products

Rabbit Recombinant Monoclonal ETS1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21909
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ETS1 also known as E26 Transformation-Specific 1 is a transcription factor protein weighing approximately 51-53 kDa. It belongs to the ETS family of transcription factors and can be referred to by other names such as ETS-1 ETS 1 or Ets-1. This protein shows expression in numerous tissues including the thymus spleen and brain. Importantly ETS1 binds to specific DNA sequences to regulate the transcription of various target genes.

Biological function summary

The function of this protein involves the regulation of gene expression linked to cell development differentiation and apoptosis. ETS1 does not form stable complexes with other proteins in its resting form but it can interact dynamically with co-factors during activation. Its activity plays a role in immune response modulation and vascular development by controlling downstream target genes connected to these processes.

Pathways

The involvement of this protein spans key signal transduction pathways including the MAPK and PI3K/AKT pathways. In these pathways ETS1 regulates gene expression influenced by external stimuli such as growth factors. It interacts with other pathway-related proteins like ERK1/2 and AKT initiating cellular responses that contribute to cell proliferation and survival mechanisms.

Associated diseases and disorders

Abnormalities in the function or expression of this protein have been linked to certain cancers such as leukemia and breast cancer. The protein interacts with other oncogenic proteins including BRISA and Palikuqi leading to progression of these malignancies. Additionally involvement in autoimmune disorders like lupus has been observed where deregulation of ETS1 can affect immune cell behavior and promote hyperactivation of immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)

    Exposure time : Lane 1: 125 seconds; Lanes 2,3 and 4: 3 minutes; Lane 5: 70 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

    Lower weak bands below 50 kDa represent other ETS1 isoforms.

    All lanes: Western blot - Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg

    Lane 2: Rat lung lysate at 20 µg

    Lane 3: Rat spleen lysate at 20 µg

    Lane 4: C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 5: Mouse lymph node lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human tonsil (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

    All lanes: Western blot - Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

    All lanes: Human lymph node lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on rat spleen (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on mouse thymus (PMID: 22307076; PMID: 3472202). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human Hodgkin's lymphoma (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com