Anti-ETS1 antibody [EPR21909]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human Hodgkin's lymphoma (PMID : 23288305; PMID : 10377039). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human tonsil (PMID : 23288305; PMID : 10377039). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on mouse thymus (PMID : 22307076; PMID : 3472202). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (AB220361)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on rat spleen (PMID : 23288305; PMID : 10377039). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-ETS1 antibody [EPR21909] (AB220361)

Exposure time : Lane 1 : 125 seconds; Lanes 2,3 and 4 : 3 minutes; Lane 5 : 70 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

Lower weak bands below 50 kDa represent other ETS1 isoforms.

All lanes:

Western blot - Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

Lane 1:

KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Lane 4:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 5:

Mouse lymph node lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-ETS1 antibody [EPR21909] (AB220361)

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.

All lanes:

Western blot - Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

All lanes:

Human lymph node lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false