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AB272866

Anti-ETS2 antibody

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(1 Publication)

Rabbit Polyclonal ETS2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein C-ets-2.

View Alternative Names

Protein C-ets-2, ETS2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody (AB272866)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody (AB272866)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ETS2 using ab272866 at 1/500 dilution (Green) in ICC/IF. Alpha-tubulin filaments (Red).

Immunoprecipitation - Anti-ETS2 antibody (AB272866)
  • IP

Supplier Data

Immunoprecipitation - Anti-ETS2 antibody (AB272866)

IP Sample : HeLa whole cell lysate/extract

7.5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-ETS2 antibody (ab272866) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 40 µg

Lane 2:

Control with 2 μg of preimmune rabbit IgG. at 40 µg

Lane 3:

Immunoprecipitation of ETS2 protein by 2 μg of ab272866 at 40 µg

Secondary

All lanes:

anti-rabbit IgG HRP

Predicted band size: 53 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody (AB272866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody (AB272866)

Paraffin-embedded rat forebrain tissue stained for ETS2 using ab272866 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

Western blot - Anti-ETS2 antibody (AB272866)
  • WB

Supplier Data

Western blot - Anti-ETS2 antibody (AB272866)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ETS2 antibody (ab272866) at 1/500 dilution

All lanes:

HeLa Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human Protein C-ets-2. The exact immunogen used to generate this antibody is proprietary information.

P15036

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Secondary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [RM1017] (AB283655)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETS2 protein or E26 transformation-specific sequence 2 is a transcription factor that is part of the ETS family. This protein is about 56 kDa in mass. It regulates gene expression by binding to specific DNA sequences. ETS2 is widely expressed in various tissues including the heart brain and liver. It influences multiple cellular processes like development proliferation and apoptosis.
Biological function summary

ETS2 protein functions in the regulation of genes that control critical cellular activities. The protein can form complexes with other transcription factors to modulate gene expression. It can interact with other proteins to influence cellular responses to external signals. As a transcription factor ETS2 also has a role in cellular differentiation and morphogenesis contributing to the development of multicellular organisms.

Pathways

ETS2 protein is involved in key signaling pathways such as the MAPK/ERK pathway and PI3K/AKT pathway. These pathways play essential roles in cell cycle regulation apoptosis and cell growth. ETS2 interacts with proteins like MAPK and AKT to transmit signals from the cell membrane to the nucleus. These interactions help coordinate responses to growth factors and stress signals influencing cellular outcomes.

The ETS2 protein has been linked to cancer and Down syndrome. In cancer dysregulation of ETS2 can lead to abnormal cell proliferation and resistance to apoptosis contributing to tumor development. In Down syndrome an extra copy of the ETS2 gene on chromosome 21 may affect normal development and contribute to associated phenotypes. ETS2's interaction with proteins like MYC and RAS can exacerbate these conditions influencing disease progression and outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.
See full target information Protein C-ets-2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e37254 PubMed39309830

2024

Knockdown of LncRNA-HAGLR restrains the viability and motility of pancreatic cancer via miR-625-5p/TAF15 axis and .

Applications

Unspecified application

Species

Unspecified reactive species

Jiafu Wang,Huiqi Gao,Peng Fu,Lin Lin,Lifan Wang,Yue Han
View all publications
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Product promise

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