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Rabbit Recombinant Monoclonal ETS2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (AB219948), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (AB219948), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (AB219948), expandable thumbnail
  • Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (AB219948), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (AB219948), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Expected
Expected
Mouse
Expected
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.

Alternative names

Protein C-ets-2, ETS2

Recommended products

Rabbit Recombinant Monoclonal ETS2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22419
Purification technique
Affinity purification Protein A
Specificity

This antibody is not recommended for human in IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ETS2 protein or E26 transformation-specific sequence 2 is a transcription factor that is part of the ETS family. This protein is about 56 kDa in mass. It regulates gene expression by binding to specific DNA sequences. ETS2 is widely expressed in various tissues including the heart brain and liver. It influences multiple cellular processes like development proliferation and apoptosis.

Biological function summary

ETS2 protein functions in the regulation of genes that control critical cellular activities. The protein can form complexes with other transcription factors to modulate gene expression. It can interact with other proteins to influence cellular responses to external signals. As a transcription factor ETS2 also has a role in cellular differentiation and morphogenesis contributing to the development of multicellular organisms.

Pathways

ETS2 protein is involved in key signaling pathways such as the MAPK/ERK pathway and PI3K/AKT pathway. These pathways play essential roles in cell cycle regulation apoptosis and cell growth. ETS2 interacts with proteins like MAPK and AKT to transmit signals from the cell membrane to the nucleus. These interactions help coordinate responses to growth factors and stress signals influencing cellular outcomes.

Associated diseases and disorders

The ETS2 protein has been linked to cancer and Down syndrome. In cancer dysregulation of ETS2 can lead to abnormal cell proliferation and resistance to apoptosis contributing to tumor development. In Down syndrome an extra copy of the ETS2 gene on chromosome 21 may affect normal development and contribute to associated phenotypes. ETS2's interaction with proteins like MYC and RAS can exacerbate these conditions influencing disease progression and outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling ETS2 with ab219948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (500 ng/ml) for 6 hours. The nuclear counterstain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    Secondary antibody contril: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)

    ETS2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab219948 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219948 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (Input).
    Lane 2: ab219948 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab219948 in K562 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The two immunoprecipitated bands represent non-phosphorylated and phosphorylated ETS2.

    All lanes: Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining on rat cerebellum (PMID: 3472202) is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining on mouse cerebrum (PMID: 3472202) is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds, Lane 2: 3 minutes.

    Lane 1 was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 2: Human thymus at 10 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 53 kDa

    Observed band size: 53 kDa

  • Western blot - Anti-ETS2 antibody [EPR22419] (ab219948), expandable thumbnail

    Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 15590657). The doublets represent ETS2 and its phosphorylated form.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

    Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 2: RAW 264.7 treated with 500ng/ml lipopolysaccharides(LPS) for 6 hours at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 53 kDa

    Observed band size: 50-55 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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