Rabbit Recombinant Monoclonal ETS2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118118.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129199.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290)")
![Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunocytochemistry-immunofluorescence-img89212.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290)")
![Immunoprecipitation - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunoprecipitation-img4622.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105844.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (AB243290)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected | Expected |
| Mouse | Expected | Not recommended | Expected | Tested | Tested |
| Rat | Predicted | Not recommended | Expected | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.
Alternative names
Protein C-ets-2, ETS2
Recommended products
Rabbit Recombinant Monoclonal ETS2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22419
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not recommended for human in IHC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243290 is the carrier-free version of Anti-ETS2 antibody [EPR22419] ab219948.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ETS2 protein or E26 transformation-specific sequence 2 is a transcription factor that is part of the ETS family. This protein is about 56 kDa in mass. It regulates gene expression by binding to specific DNA sequences. ETS2 is widely expressed in various tissues including the heart brain and liver. It influences multiple cellular processes like development proliferation and apoptosis.
- Biological function summary
ETS2 protein functions in the regulation of genes that control critical cellular activities. The protein can form complexes with other transcription factors to modulate gene expression. It can interact with other proteins to influence cellular responses to external signals. As a transcription factor ETS2 also has a role in cellular differentiation and morphogenesis contributing to the development of multicellular organisms.
- Pathways
ETS2 protein is involved in key signaling pathways such as the MAPK/ERK pathway and PI3K/AKT pathway. These pathways play essential roles in cell cycle regulation apoptosis and cell growth. ETS2 interacts with proteins like MAPK and AKT to transmit signals from the cell membrane to the nucleus. These interactions help coordinate responses to growth factors and stress signals influencing cellular outcomes.
- Associated diseases and disorders
The ETS2 protein has been linked to cancer and Down syndrome. In cancer dysregulation of ETS2 can lead to abnormal cell proliferation and resistance to apoptosis contributing to tumor development. In Down syndrome an extra copy of the ETS2 gene on chromosome 21 may affect normal development and contribute to associated phenotypes. ETS2's interaction with proteins like MYC and RAS can exacerbate these conditions influencing disease progression and outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118118.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS2 with Anti-ETS2 antibody [EPR22419] ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129199.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling ETS2 with Anti-ETS2 antibody [EPR22419] ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).![Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunocytochemistry-immunofluorescence-img89212.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling ETS2 with Anti-ETS2 antibody [EPR22419] ab219948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (500 ng/ml) for 6 hours. The nuclear counterstain is DAPI (blue).
Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
Secondary antibody contril: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).
![Immunoprecipitation - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunoprecipitation-img4622.jpg "Immunoprecipitation - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunoprecipitation - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
ETS2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with Anti-ETS2 antibody [EPR22419] ab219948 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-ETS2 antibody [EPR22419] ab219948 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: K562 whole cell lysate 10 μg (Input).
Lane 2: Anti-ETS2 antibody [EPR22419] ab219948 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ETS2 antibody [EPR22419] ab219948 in K562 whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.The two immunoprecipitated bands represent non-phosphorylated and phosphorylated ETS2.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).
All lanes: Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (Anti-ETS2 antibody [EPR22419] ab219948)
Predicted band size: 53 kDa
Observed band size: 53 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105844.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ETS2 with Anti-ETS2 antibody [EPR22419] ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining on rat cerebellum (PMID: 3472202) is observed. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80796.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ETS2 with Anti-ETS2 antibody [EPR22419] ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining on mouse cerebrum (PMID: 3472202) is observed. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ETS2 antibody [EPR22419] ab219948).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290), expandable thumbnail](https://content.abcam.com/products/images/ets2-antibody-epr22419-bsa-and-azide-free-ab243290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80796.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] - BSA and Azide free (ab243290)")
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