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Rabbit Recombinant Monoclonal ETV2 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell lysate - Mouse, Transfected cell line - Human samples. Cited in 7 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Transfected cell line - Human
Not recommended
Tested
Transfected cell lysate - Human
Tested
Not recommended
Transfected cell lysate - Mouse
Tested
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000 - 1/10000

Notes

-

Species

Transfected cell lysate - Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Transfected cell lysate - Mouse

Dilution info

-

Notes

-

Target data

Function

Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal ETV2 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell lysate - Mouse, Transfected cell line - Human samples. Cited in 7 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR5229(3)

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

ETV2 regulates endothelial cell development and vasculogenesis. It acts as an important switch for endothelial cell fate essentially directing stem cells towards an endothelial lineage. ETV2 often operates within a larger protein complex interacting with other transcription factors necessary for vascular differentiation. Its role indicates a pivotal function in blood vessel formation and remodeling greatly influencing vascular health.

Mechanical summary

ETV2 also known as ETS variant transcription factor 2 functions as a transcription factor important in the regulation of gene expression involved with endothelial lineage differentiation. This protein has a mass of approximately 38 kDa. ETV2 is mainly expressed in embryonic vascular endothelial cells as well as in adult tissues like the heart and lungs. Some studies also refer to it by the alternate name ER71. High expression levels in these key areas support its critical roles in vasculature development and function.

Pathway

ETV2 is a part of the VEGF (vascular endothelial growth factor) signaling pathway and the NOTCH signaling pathway. In the VEGF pathway ETV2 directly influences genes responsible for angiogenesis the process of forming new blood vessels. The NOTCH pathway involves ETV2 in the differentiation and proliferation of endothelial cells. ETV2 interacts with proteins such as VEGFA and NOTCH1 both significant contributors to vascular biology and embryonic development.

Disease

ETV2 relates to cardiovascular diseases and certain cancers. Dysregulation of ETV2 expression can contribute to impaired blood vessel formation playing a part in conditions like atherosclerosis and peripheral artery disease. The link between ETV2 and cancers such as breast cancer reveals its involvement in tumor angiogenesis as tumors harness ETV2 to recruit blood vessels promoting their growth. ETV2 also connects to the protein HIF1A which plays a critical role in cancer angiogenesis under low oxygen conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ETV2 antibody [EPR5229(3)] (ab181847), expandable thumbnail

    Western blot - Anti-ETV2 antibody [EPR5229(3)] (ab181847)

    All lanes: Western blot - Anti-ETV2 antibody [EPR5229(3)] (AB181847) at 1/10000 dilution

    Lane 1: HUVEC cell lysate

    Lane 2: HUVEC cell lysate of lent-virally expressed Human ETV2

    Lane 3: Human bone marrow stromal cell lysate

    Lane 4: Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2

    Developed using the ECL technique.

    Predicted band size: 37 kDa

    Observed band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] (ab181847), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] (ab181847)

    Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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