AB181847

Anti-ETV2 antibody [EPR5229(3)]

RabMAb
Recombinant
What is this?

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(13 Publications)

Anti-ETV2 antibody [EPR5229(3)] (ab181847) is a rabbit monoclonal antibody detecting ETV2 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ER71, ETSRP71, ETV2, ETS translocation variant 2, Ets-related protein 71

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] (AB181847)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] (AB181847)

Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

Supplier Data

Western blot - Anti-ETV2 antibody [EPR5229(3)] (AB181847)

All lanes:

Western blot - Anti-ETV2 antibody [EPR5229(3)] (ab181847) at 1/10000 dilution

Lane 1:

HUVEC cell lysate

Lane 2:

HUVEC cell lysate of lent-virally expressed Human ETV2

Lane 3:

Human bone marrow stromal cell lysate

Lane 4:

Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2

Predicted band size: 37 kDa

Observed band size: 40 kDa

true

Carrier free

Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free
660 APC

APC Anti-ETV2 antibody [EPR5229(3)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ETV2 antibody [EPR5229(3)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ETV2 antibody [EPR5229(3)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ETV2 antibody [EPR5229(3)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ETV2 antibody [EPR5229(3)]
578 PE

PE Anti-ETV2 antibody [EPR5229(3)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5229(3)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ETV2 antibody [EPR5229(3)] (ab181847) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of ETV2?
Anti-ETV2 [EPR5229(3)] (ab181847) specifically detects a band for ETV2 (UniProt: O00321) at a molecular weight of 37kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5229(3)] also available for your convenience: ab181847, Carrier free - ab250521, APC - ab319250, PE - ab319398, Alexa Fluor® 488 - ab319532, Alexa Fluor® 647 - ab319645, Alexa Fluor® 594 - ab319749, Alexa Fluor® 555 - ab319891

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETV2 also known as ETS variant transcription factor 2 functions as a transcription factor important in the regulation of gene expression involved with endothelial lineage differentiation. This protein has a mass of approximately 38 kDa. ETV2 is mainly expressed in embryonic vascular endothelial cells as well as in adult tissues like the heart and lungs. Some studies also refer to it by the alternate name ER71. High expression levels in these key areas support its critical roles in vasculature development and function.

Biological function summary

ETV2 regulates endothelial cell development and vasculogenesis. It acts as an important switch for endothelial cell fate essentially directing stem cells towards an endothelial lineage. ETV2 often operates within a larger protein complex interacting with other transcription factors necessary for vascular differentiation. Its role indicates a pivotal function in blood vessel formation and remodeling greatly influencing vascular health.

Pathways

ETV2 is a part of the VEGF (vascular endothelial growth factor) signaling pathway and the NOTCH signaling pathway. In the VEGF pathway ETV2 directly influences genes responsible for angiogenesis the process of forming new blood vessels. The NOTCH pathway involves ETV2 in the differentiation and proliferation of endothelial cells. ETV2 interacts with proteins such as VEGFA and NOTCH1 both significant contributors to vascular biology and embryonic development.

ETV2 relates to cardiovascular diseases and certain cancers. Dysregulation of ETV2 expression can contribute to impaired blood vessel formation playing a part in conditions like atherosclerosis and peripheral artery disease. The link between ETV2 and cancers such as breast cancer reveals its involvement in tumor angiogenesis as tumors harness ETV2 to recruit blood vessels promoting their growth. ETV2 also connects to the protein HIF1A which plays a critical role in cancer angiogenesis under low oxygen conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.

See full target information ETV2

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 36:102592 PubMed40612712

2025

mRNA-based induction of ETV2 to restore blood flow in a preclinical mouse hindlimb ischemia model.

Applications

Unspecified application

Species

Unspecified reactive species

Hanae Toyonaga,Kazunori Arai,Mengdi Zhang,Lei Cheng,Hirotsugu Tanaka

iScience 28:111538 PubMed39811655

2025

ETV2/ER71 regulates hematovascular lineage generation and vascularization through an H3K9 demethylase, KDM4A.

Applications

Unspecified application

Species

Unspecified reactive species

Min Seong Kim,Raham Lee,Dong Hun Lee,Heesang Song,Taekyung Ha,Joo Kyung Kim,Bum-Yong Kang,Karl Agger,Kristian Helin,Donghyun Shin,Yunhee Kang,Changwon Park

Nature cardiovascular research 4:45-63 PubMed39747454

2025

Direct specification of lymphatic endothelium from mesenchymal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Irina-Elena Lupu,David E Grainger,Nils Kirschnick,Sarah Weischer,Erica Zhao,Ines Martinez-Corral,Hans Schoofs,Marie Vanhollebeke,Grace Jones,Jonathan Godwin,Aden Forrow,Ines Lahmann,Paul R Riley,Thomas Zobel,Kari Alitalo,Taija Mäkinen,Friedemann Kiefer,Oliver A Stone

Bioactive materials 37:222-238 PubMed38549772

2024

ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

HaoRan Du,Bang Li,Rui Yu,Xiaoxuan Lu,ChengLin Li,HuiHui Zhang,Fan Yang,RongQuan Zhao,WeiMin Bao,Xuan Yin,YuanYin Wang,Jian Zhou,Jianguang Xu

JCI insight 9: PubMed38456502

2024

CDK1 inhibition reduces osteogenesis in endothelial cells in vascular calcification.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Yang Yang,Xiuju Wu,Li Zhang,Xinjiang Cai,Jaden Ji,Sydney Chen,Abigail Vera,Kristina I Boström,Yucheng Yao

STAR protocols 4:102292 PubMed37149860

2023

Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tea Soon Park,Rishabh Hirday,Amir Ali,Roba Megersa,Rafael Villasmil,Eric Nguyen,Kapil Bharti

Frontiers in cell and developmental biology 11:1109648 PubMed36923254

2023

ETV2 and VEZF1 interaction and regulation of the hematoendothelial lineage during embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Satyabrata Das,Vinayak Gupta,Johannes Bjorge,Xiaozhong Shi,Wuming Gong,Mary G Garry,Daniel J Garry

Cell proliferation 56:e13366 PubMed36478274

2022

CD34 cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Soo-Been Jeon,A-Reum Han,Sunghun Lee,Seung Chan Lee,Min Ji Lee,Soon-Jung Park,Sung-Hwan Moon,Ji Yoon Lee

Cancers 14: PubMed35053574

2022

Introduction of Mutant GNAQ into Endothelial Cells Induces a Vascular Malformation Phenotype with Therapeutic Response to Imatinib.

Applications

Unspecified application

Species

Unspecified reactive species

Maiko Sasaki,Yoonhee Jung,Paula North,Justin Elsey,Keith Choate,Michael Andrew Toussaint,Christina Huang,Rakan Radi,Adam J Perricone,Victor G Corces,Jack L Arbiser

Frontiers in genetics 12:616507 PubMed33732284

2021

Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Tan,Hecheng Zhu,Guihua Tang,Hongwei Liu,Siyi Wanggou,Yudong Cao,Zhaoqi Xin,Quanwei Zhou,Chaohong Zhan,Zhaoping Wu,Youwei Guo,Zhipeng Jiang,Ming Zhao,Caiping Ren,Xingjun Jiang,Wen Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETV2 antibody [EPR5229(3)]

Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] (AB181847)

Western blot - Anti-ETV2 antibody [EPR5229(3)] (AB181847)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise