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AB250521

Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ETV2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

ER71, ETSRP71, ETV2, ETS translocation variant 2, Ets-related protein 71

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (AB250521)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (AB250521)

Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181847).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5229(3)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab250521 is the carrier-free version of ab181847.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETV2 also known as ETS variant transcription factor 2 functions as a transcription factor important in the regulation of gene expression involved with endothelial lineage differentiation. This protein has a mass of approximately 38 kDa. ETV2 is mainly expressed in embryonic vascular endothelial cells as well as in adult tissues like the heart and lungs. Some studies also refer to it by the alternate name ER71. High expression levels in these key areas support its critical roles in vasculature development and function.
Biological function summary

ETV2 regulates endothelial cell development and vasculogenesis. It acts as an important switch for endothelial cell fate essentially directing stem cells towards an endothelial lineage. ETV2 often operates within a larger protein complex interacting with other transcription factors necessary for vascular differentiation. Its role indicates a pivotal function in blood vessel formation and remodeling greatly influencing vascular health.

Pathways

ETV2 is a part of the VEGF (vascular endothelial growth factor) signaling pathway and the NOTCH signaling pathway. In the VEGF pathway ETV2 directly influences genes responsible for angiogenesis the process of forming new blood vessels. The NOTCH pathway involves ETV2 in the differentiation and proliferation of endothelial cells. ETV2 interacts with proteins such as VEGFA and NOTCH1 both significant contributors to vascular biology and embryonic development.

ETV2 relates to cardiovascular diseases and certain cancers. Dysregulation of ETV2 expression can contribute to impaired blood vessel formation playing a part in conditions like atherosclerosis and peripheral artery disease. The link between ETV2 and cancers such as breast cancer reveals its involvement in tumor angiogenesis as tumors harness ETV2 to recruit blood vessels promoting their growth. ETV2 also connects to the protein HIF1A which plays a critical role in cancer angiogenesis under low oxygen conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.
See full target information ETV2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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