AB176717

Anti-ETV3 antibody

Name: Anti-ETV3 antibody (ab176717) | Abcam
Brand: Abcam Limited
SKU: AB176717

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(2 Publications)

Rabbit Polyclonal ETV3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ETV3 aa 450-500.

View Alternative Names

METS, PE1, ETV3, ETS translocation variant 3, ETS domain transcriptional repressor PE1, Mitogenic Ets transcriptional suppressor, PE-1

2 Images

Supplier Data

Immunoprecipitation - Anti-ETV3 antibody (AB176717)

Detection of ETV3 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176717 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ETV3 antibody (ab176717)

Predicted band size: 57 kDa

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Supplier Data

Western blot - Anti-ETV3 antibody (AB176717)

All lanes:

Western blot - Anti-ETV3 antibody (ab176717) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 57 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ETV3 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

P41162

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176717 was affinity purified using an epitope specific to ETV3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETV3 also known as ETS Variant 3 functions as a transcriptional repressor. The protein has a mass of approximately 61 kDa. It is expressed in multiple tissues including skin liver and immune cells. The presence of ETV3 is important for regulating gene expression as it can bind to specific DNA sequences and inhibit transcription of target genes. This ability allows ETV3 to maintain control over cellular processes such as proliferation and differentiation.

Biological function summary

The functionality of ETV3 extends to modulation of immune responses and cellular development. ETV3 along with other transcription factors forms parts of multi-protein complexes. Its role in these complexes involves the repression of gene activity directly influencing cell cycle progression and immune system activity. ETV3’s expression and interactions suggest it coordinates with other molecules to maintain homeostasis and appropriate cellular functions.

Pathways

ETV3 integrates into two main biological pathways: the MAPK signaling pathway and immune response regulation. Within the MAPK pathway ETV3 interacts with proteins such as ERK influencing cell survival and differentiation outcomes. Meanwhile it connects in immune pathways by modulating activity of other transcription factors impacting immune cell behaviors and responses.

ETV3 plays a role in conditions like skin cancer and immune system diseases. Aberrant expression or mutation of ETV3 correlates with melanoma development where disrupted regulation contributes to abnormal cell proliferation. Its interactions with proteins such as MITF are particularly relevant in melanoma. Additionally ETV3's role in immune modulation can influence autoimmune disorders where dysregulation leads to improper immune activation or repression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.

See full target information ETV3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:96 PubMed39285160

2024

Targeting SNRNP200-induced splicing dysregulation offers an immunotherapy opportunity for glycolytic triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiao Yang,Luo Hong,Linwei Guo,Yunjin Wang,Xiangchen Han,Boyue Han,Zheng Xing,Guoliang Zhang,Hongxia Zhou,Chao Chen,Hong Ling,Zhimin Shao,Xin Hu

Arthritis & rheumatology (Hoboken, N.J.) 74:671-681 PubMed34652073

2022

Identification of Novel Risk Loci for Behçet's Disease-Related Uveitis in a Chinese Population in a Genome-Wide Association Study.

Applications

Unspecified application

Species

Unspecified reactive species

Guannan Su,Zhenyu Zhong,Qingyun Zhou,Liping Du,Zi Ye,Fuzhen Li,Wenjuan Zhuang,Chaokui Wang,Liang Liang,Yan Ji,Qingfeng Cao,Qingfeng Wang,Rui Chang,Handan Tan,Shenglan Yi,Yujing Li,Xiaojie Feng,Weiting Liao,Wanyun Zhang,Jia Shu,Shiyao Tan,Jing Xu,Su Pan,Hongxi Li,Jing Shi,Zhijun Chen,Ying Zhu,Xingsheng Ye,Xiao Tan,Jun Zhang,Zhangluxi Liu,Fanfan Huang,Gangxiang Yuan,Tingting Pang,Yizong Liu,Jiadong Ding,Yingnan Gao,Meifen Zhang,Wei Chi,Xiaoli Liu,Yuqin Wang,Ling Chen,Akira Meguro,Masaki Takeuchi,Nobuhisa Mizuki,Shigeaki Ohno,Xianbo Zuo,Aize Kijlstra,Peizeng Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ETV3 antibody

Immunoprecipitation - Anti-ETV3 antibody (AB176717)

Western blot - Anti-ETV3 antibody (AB176717)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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