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AB154810

Anti-ETV3 antibody [EPR8658]

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(1 Publication)

Rabbit Recombinant Monoclonal ETV3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

METS, PE1, ETV3, ETS translocation variant 3, ETS domain transcriptional repressor PE1, Mitogenic Ets transcriptional suppressor, PE-1

2 Images
Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (AB154810)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (AB154810)

Intracellular flow cytometric analysis of permeabilized Hela cells labeling ETV3 with ab154810 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

Western blot - Anti-ETV3 antibody [EPR8658] (AB154810)
  • WB

Unknown

Western blot - Anti-ETV3 antibody [EPR8658] (AB154810)

All lanes:

Western blot - Anti-ETV3 antibody [EPR8658] (ab154810) at 1/10000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

SHSY5Y lysate at 10 µg

Lane 4:

U937 lysate at 10 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8658

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETV3 also known as ETS Variant 3 functions as a transcriptional repressor. The protein has a mass of approximately 61 kDa. It is expressed in multiple tissues including skin liver and immune cells. The presence of ETV3 is important for regulating gene expression as it can bind to specific DNA sequences and inhibit transcription of target genes. This ability allows ETV3 to maintain control over cellular processes such as proliferation and differentiation.
Biological function summary

The functionality of ETV3 extends to modulation of immune responses and cellular development. ETV3 along with other transcription factors forms parts of multi-protein complexes. Its role in these complexes involves the repression of gene activity directly influencing cell cycle progression and immune system activity. ETV3’s expression and interactions suggest it coordinates with other molecules to maintain homeostasis and appropriate cellular functions.

Pathways

ETV3 integrates into two main biological pathways: the MAPK signaling pathway and immune response regulation. Within the MAPK pathway ETV3 interacts with proteins such as ERK influencing cell survival and differentiation outcomes. Meanwhile it connects in immune pathways by modulating activity of other transcription factors impacting immune cell behaviors and responses.

ETV3 plays a role in conditions like skin cancer and immune system diseases. Aberrant expression or mutation of ETV3 correlates with melanoma development where disrupted regulation contributes to abnormal cell proliferation. Its interactions with proteins such as MITF are particularly relevant in melanoma. Additionally ETV3's role in immune modulation can influence autoimmune disorders where dysregulation leads to improper immune activation or repression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.
See full target information ETV3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35008858

2021

New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique de Seny,Dominique Baiwir,Elettra Bianchi,Gaël Cobraiville,Céline Deroyer,Christophe Poulet,Olivier Malaise,Geneviève Paulissen,Marie-Joëlle Kaiser,Jean-Philippe Hauzeur,Gabriel Mazzucchelli,Philippe Delvenne,Michel Malaise
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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