AB315973

Anti-EVI1 antibody [EPR26816-18]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal EVI1 antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Evi1, Mds1, Prdm3, Mecom, Histone-lysine N-methyltransferase MECOM, Ecotropic virus integration site 1 protein, MDS1 and EVI1 complex locus protein, Myelodysplasia syndrome 1 protein homolog, EVI-1

3 Images

Supplier Data

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : WEHI-231 (PMID : 2842066).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins : EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID : (PMID : 24944602).

The identity of the higher MW band at approximately 300 kDa is unknown.

In Western blot, Anti-Lamin B1 antibody [EPR8985 (B)] (ab133741) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

Lane 1:

MEF (mouse embryo fibroblast) whole cell lysate at 60 µg

Lane 2:

WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 75 kDa,200 kDa,70 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HL-1.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins : EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID : (PMID : 24944602).

The identity of the bands between 75 kDa and 180 kDa are unknown.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 80 µg

Lane 2:

HL-1 (mouse atrial muscle cell) whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa,125 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins : EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID : (PMID : 24944602).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) nuclear fraction at 20 µg

Lane 2:

NIH/3T3 cytoplasmic fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 145 kDa,200 kDa,36 kDa,15 kDa

false

Exposure time: 169s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26816-18

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EVI1 also known as MECOM or MECOM way is a transcription factor with a molecular mass of approximately 145 kDa. It is primarily expressed in hematopoietic stem cells and various tissues including lung and kidney. EVI1 plays a role in regulating gene expression by binding to specific DNA sequences influencing the transcription of target genes. Its expression is tightly controlled during development and aberrant expression frequently correlates with pathological conditions.

Biological function summary

EVI1 participates in the regulation of cell growth and differentiation. As part of a complex with other proteins EVI1 modulates transcriptional outcomes necessary for maintaining stem cell identity and preventing premature differentiation. It helps balance cell cycle progression and apoptosis impacting cellular responses to external stimuli. EVI1 cooperates with other transcription factors in gene regulation networks that control developmental processes.

Pathways

EVI1 interacts with several signaling pathways notably the TGF-beta and MAPK pathways. It influences TGF-beta signaling by modulating transcription factors such as SMADs which are central to this pathway. EVI1's association with MAPK signaling impacts cell proliferation and differentiation integrating multiple signaling inputs to coordinate cellular outcomes. Through these pathways EVI1 interacts with proteins like SMAD3 and ERK essential players in its regulatory circuit.

EVI1 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). In AML aberrant expression or rearrangements involving EVI1 result in blockages of normal myeloid differentiation leading to leukemic transformation. EVI1 also associates with transcription factors like GATA2 in these diseases intensifying the disruption of normal hematopoietic functions. Understanding EVI1's role offers insights into the molecular mechanisms underlying these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.. Isoform 3. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.

See full target information Mecom

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EVI1 antibody [EPR26816-18]

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise