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Rabbit Recombinant Monoclonal EVI1 antibody. Suitable for WB and reacts with Mouse samples.

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Images

Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973), expandable thumbnail
  • Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973), expandable thumbnail
  • Western blot - Anti-EVI1 antibody [EPR26816-18] (AB315973), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Isoform 1. Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis. Isoform 3. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.

Alternative names

Evi1, Mds1, Prdm3, Mecom, Histone-lysine N-methyltransferase MECOM, Ecotropic virus integration site 1 protein, MDS1 and EVI1 complex locus protein, Myelodysplasia syndrome 1 protein homolog, EVI-1

Recommended products

Rabbit Recombinant Monoclonal EVI1 antibody. Suitable for WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26816-18
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EVI1 also known as MECOM or MECOM way is a transcription factor with a molecular mass of approximately 145 kDa. It is primarily expressed in hematopoietic stem cells and various tissues including lung and kidney. EVI1 plays a role in regulating gene expression by binding to specific DNA sequences influencing the transcription of target genes. Its expression is tightly controlled during development and aberrant expression frequently correlates with pathological conditions.

Biological function summary

EVI1 participates in the regulation of cell growth and differentiation. As part of a complex with other proteins EVI1 modulates transcriptional outcomes necessary for maintaining stem cell identity and preventing premature differentiation. It helps balance cell cycle progression and apoptosis impacting cellular responses to external stimuli. EVI1 cooperates with other transcription factors in gene regulation networks that control developmental processes.

Pathways

EVI1 interacts with several signaling pathways notably the TGF-beta and MAPK pathways. It influences TGF-beta signaling by modulating transcription factors such as SMADs which are central to this pathway. EVI1's association with MAPK signaling impacts cell proliferation and differentiation integrating multiple signaling inputs to coordinate cellular outcomes. Through these pathways EVI1 interacts with proteins like SMAD3 and ERK essential players in its regulatory circuit.

Associated diseases and disorders

EVI1 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). In AML aberrant expression or rearrangements involving EVI1 result in blockages of normal myeloid differentiation leading to leukemic transformation. EVI1 also associates with transcription factors like GATA2 in these diseases intensifying the disruption of normal hematopoietic functions. Understanding EVI1's role offers insights into the molecular mechanisms underlying these malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973), expandable thumbnail

    Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973)

    Low expression: HL-1.

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).

    The identity of the bands between 75 kDa and 180 kDa are unknown.

    In Western blot, Anti-Integrin alpha V antibody [EPR16800] (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 80 µg

    Lane 2: HL-1 (mouse atrial muscle cell) whole cell lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa, 125 kDa

    Exposure time: 59s

  • Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973), expandable thumbnail

    Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973)

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) nuclear fraction at 20 µg

    Lane 2: NIH/3T3 cytoplasmic fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 145 kDa, 200 kDa, 36 kDa, 15 kDa

    Exposure time: 169s

  • Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973), expandable thumbnail

    Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973)

    Negative control: WEHI-231 (PMID:2842066).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).

    The identity of the higher MW band at approximately 300 kDa is unknown.

    In Western blot, Anti-Lamin B1 antibody [EPR8985 (B)] (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (ab315973) at 1/1000 dilution

    Lane 1: MEF (mouse embryo fibroblast) whole cell lysate at 60 µg

    Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 60 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Observed band size: 75 kDa, 200 kDa, 70 kDa

    Exposure time: 59s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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