AB248063

Anti-EVI1 antibody [EPR5996] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal EVI1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EVI1, MDS1, PRDM3, MECOM, Histone-lysine N-methyltransferase MECOM, Ecotropic virus integration site 1 protein homolog, MDS1 and EVI1 complex locus protein, Myelodysplasia syndrome 1 protein, Myelodysplasia syndrome-associated protein 1, EVI-1

1 Images

Unknown

Western blot - Anti-EVI1 antibody [EPR5996] - BSA and Azide free (AB248063)

This data was developed using ab124934, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EVI1 antibody [EPR5996] (<a href='/en-us/products/primary-antibodies/evi1-antibody-epr5996-ab124934'>ab124934</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SKOV 3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit at 1/2000 dilution

Predicted band size: 118 kDa

Observed band size: 105 kDa,145 kDa,200 kDa

false

Unconjugated

Anti-EVI1 antibody [EPR5996]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5996

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab248063 is the carrier-free version of ab124934.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EVI1 also known as MECOM or MECOM way is a transcription factor with a molecular mass of approximately 145 kDa. It is primarily expressed in hematopoietic stem cells and various tissues including lung and kidney. EVI1 plays a role in regulating gene expression by binding to specific DNA sequences influencing the transcription of target genes. Its expression is tightly controlled during development and aberrant expression frequently correlates with pathological conditions.

Biological function summary

EVI1 participates in the regulation of cell growth and differentiation. As part of a complex with other proteins EVI1 modulates transcriptional outcomes necessary for maintaining stem cell identity and preventing premature differentiation. It helps balance cell cycle progression and apoptosis impacting cellular responses to external stimuli. EVI1 cooperates with other transcription factors in gene regulation networks that control developmental processes.

Pathways

EVI1 interacts with several signaling pathways notably the TGF-beta and MAPK pathways. It influences TGF-beta signaling by modulating transcription factors such as SMADs which are central to this pathway. EVI1's association with MAPK signaling impacts cell proliferation and differentiation integrating multiple signaling inputs to coordinate cellular outcomes. Through these pathways EVI1 interacts with proteins like SMAD3 and ERK essential players in its regulatory circuit.

EVI1 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). In AML aberrant expression or rearrangements involving EVI1 result in blockages of normal myeloid differentiation leading to leukemic transformation. EVI1 also associates with transcription factors like GATA2 in these diseases intensifying the disruption of normal hematopoietic functions. Understanding EVI1's role offers insights into the molecular mechanisms underlying these malignancies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Isoform 1. Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.. Isoform 7. Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.

See full target information MECOM

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EVI1 antibody [EPR5996] - BSA and Azide free

Western blot - Anti-EVI1 antibody [EPR5996] - BSA and Azide free (AB248063)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise