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AB204237

Anti-EVPL antibody

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(2 Publications)

Rabbit Polyclonal EVPL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EVPL aa 100-250.

View Alternative Names

Envoplakin, 210 kDa cornified envelope precursor protein, 210 kDa paraneoplastic pemphigus antigen, p210, EVPL

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (AB204237)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (AB204237)

Immunohistochemical analysis of human skin tissue labeling EVPL (high expression) with ab204237 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EVPL antibody (AB204237)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EVPL antibody (AB204237)

Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling EVPL in the intermediate filaments with ab204237 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (AB204237)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (AB204237)

Immunohistochemical analysis of human skeletal muscle tissue labeling EVPL (low expression) with ab204237 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EVPL aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q92817

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EVPL also known as Envoplakin is a protein with a molecular weight of approximately 201 kDa. It plays a mechanical role in forming the cornified envelope in keratinocytes providing structural integrity and barrier function to the skin. EVPL constitutes one of the proteins in the plakin family. It predominantly expresses in epithelial tissues especially in the stratified squamous epithelia including skin and mucosal surfaces.
Biological function summary

The EVPL protein contributes to cell-cell adhesion and cytoskeletal organization. It functions as a linker protein that anchors intermediate filaments to desmosomes which are cell structures that maintain tissue integrity. As a component of the desmosome complex EVPL interacts with other plakins and proteins such as periplakin and desmoplakin which together ensure the mechanical stability of cells in tissues.

Pathways

Envoplakin plays an important role in the cornified envelope formation pathway and the cell adhesion pathways. It engages with proteins like involucrin and loricrin which are essential for cross-linking and stabilizing the extracellular matrix within the skin layers. This activity helps in maintaining skin elasticity and resilience against mechanical stress.

EVPL has associations with skin disorders such as palmoplantar keratoderma and pemphigus. In these conditions structural defects in desmosomes or associated components lead to compromised cell-cell adhesion. Additionally the protein can link to desmoplakin which is involved in similar diseases demonstrating its significant impact on maintaining skin health and integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
See full target information EVPL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 82:103613 PubMed40174478

2025

Repression of oxidative phosphorylation by NR2F2, MTERF3 and GDF15 in human skin under high-glucose stress.

Applications

Unspecified application

Species

Unspecified reactive species

S Ley-Ngardigal,S Claverol,L Sobilo,M Moreau,C Hubert,J Goupil,A Poulignon,W Mahfouf,H Fatrouni,L Dard,M Juan,L Gales,A Merched,C Tokarski,E Leblanc,A Galinier,D Lacombe,H R Rezvani,F Bellvert,K Pays,C Nizard,N Dias Amoedo,A L Bulteau,R Rossignol

Journal of inflammation research 17:1687-1706 PubMed38504693

2024

Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Cai,Minliang Chen
View all publications
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Product promise

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