Rabbit Polyclonal EVPL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EVPL aa 100-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EVPL aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q92817
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
Alternative names
Envoplakin, 210 kDa cornified envelope precursor protein, 210 kDa paraneoplastic pemphigus antigen, p210, EVPL
Recommended products
Rabbit Polyclonal EVPL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EVPL aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EVPL aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q92817
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EVPL also known as Envoplakin is a protein with a molecular weight of approximately 201 kDa. It plays a mechanical role in forming the cornified envelope in keratinocytes providing structural integrity and barrier function to the skin. EVPL constitutes one of the proteins in the plakin family. It predominantly expresses in epithelial tissues especially in the stratified squamous epithelia including skin and mucosal surfaces.
- Biological function summary
The EVPL protein contributes to cell-cell adhesion and cytoskeletal organization. It functions as a linker protein that anchors intermediate filaments to desmosomes which are cell structures that maintain tissue integrity. As a component of the desmosome complex EVPL interacts with other plakins and proteins such as periplakin and desmoplakin which together ensure the mechanical stability of cells in tissues.
- Pathways
Envoplakin plays an important role in the cornified envelope formation pathway and the cell adhesion pathways. It engages with proteins like involucrin and loricrin which are essential for cross-linking and stabilizing the extracellular matrix within the skin layers. This activity helps in maintaining skin elasticity and resilience against mechanical stress.
- Associated diseases and disorders
EVPL has associations with skin disorders such as palmoplantar keratoderma and pemphigus. In these conditions structural defects in desmosomes or associated components lead to compromised cell-cell adhesion. Additionally the protein can link to desmoplakin which is involved in similar diseases demonstrating its significant impact on maintaining skin health and integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (ab204237)
Immunohistochemical analysis of human skin tissue labeling EVPL (high expression) with ab204237 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-EVPL antibody (ab204237)
Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling EVPL in the intermediate filaments with ab204237 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody (ab204237)
Immunohistochemical analysis of human skeletal muscle tissue labeling EVPL (low expression) with ab204237 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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