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AB242119

Anti-EVPL antibody

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(1 Publication)

Rabbit Polyclonal EVPL antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EVPL aa 1950 to C-terminus.

View Alternative Names

Envoplakin, 210 kDa cornified envelope precursor protein, 210 kDa paraneoplastic pemphigus antigen, p210, EVPL

1 Images
Immunoprecipitation - Anti-EVPL antibody (AB242119)
  • IP

Supplier Data

Immunoprecipitation - Anti-EVPL antibody (AB242119)

EVPL was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab242119 used for IP at 8 μl per reaction. For WB at a 1 : 400 dilution.

Lane 1 : ab242119 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 3 minutes.

All lanes:

Immunoprecipitation - Anti-EVPL antibody (ab242119)

Predicted band size: 232 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human EVPL aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92817

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>" } } }
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Product details

ab242119 has not performed satisfactorily when used for WB of EVPL in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of EVPL.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to EVPL immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EVPL also known as Envoplakin is a protein with a molecular weight of approximately 201 kDa. It plays a mechanical role in forming the cornified envelope in keratinocytes providing structural integrity and barrier function to the skin. EVPL constitutes one of the proteins in the plakin family. It predominantly expresses in epithelial tissues especially in the stratified squamous epithelia including skin and mucosal surfaces.
Biological function summary

The EVPL protein contributes to cell-cell adhesion and cytoskeletal organization. It functions as a linker protein that anchors intermediate filaments to desmosomes which are cell structures that maintain tissue integrity. As a component of the desmosome complex EVPL interacts with other plakins and proteins such as periplakin and desmoplakin which together ensure the mechanical stability of cells in tissues.

Pathways

Envoplakin plays an important role in the cornified envelope formation pathway and the cell adhesion pathways. It engages with proteins like involucrin and loricrin which are essential for cross-linking and stabilizing the extracellular matrix within the skin layers. This activity helps in maintaining skin elasticity and resilience against mechanical stress.

EVPL has associations with skin disorders such as palmoplantar keratoderma and pemphigus. In these conditions structural defects in desmosomes or associated components lead to compromised cell-cell adhesion. Additionally the protein can link to desmoplakin which is involved in similar diseases demonstrating its significant impact on maintaining skin health and integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
See full target information EVPL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:22 PubMed38989294

2024

Mitochondrial protein isoleucyl-tRNA synthetase 2 in tumor cells as a potential therapeutic target for cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojiao Meng,Bo Gao,Ning Li
View all publications
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Product promise

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