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AB252829

Anti-EWSR1/EWS antibody [21B1]

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(3 Publications)

Rat Recombinant Monoclonal EWSR1/EWS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

EWS, EWSR1, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein

3 Images
Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (AB252829)
  • IP

Supplier Data

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (AB252829)

EWSR1/EWS was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252829 at 12.03μg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab252829 IP in HeLa whole cell lysate.
Lane 3 : Rat monoclonal IgG instead of ab252829 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 secs.

All lanes:

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)

Predicted band size: 68 kDa

false

Exposure time: 3s

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (AB252829)
  • IP

Supplier Data

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (AB252829)

EWSR1/EWS was immunoprecipitated from K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252829 at 12.03μg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1 : K562 whole cell lysate 10μg (Input).
Lane 2 : ab252829 IP in K562 whole cell lysate.
Lane 3 : Rat monoclonal IgG instead of ab252829 in K562 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 secs.

All lanes:

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)

Predicted band size: 68 kDa

false

Exposure time: 3s

Western blot - Anti-EWSR1/EWS antibody [21B1] (AB252829)
  • WB

Supplier Data

Western blot - Anti-EWSR1/EWS antibody [21B1] (AB252829)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 15 secs.

All lanes:

Western blot - Anti-EWSR1/EWS antibody [21B1] (ab252829) at 0.361 µg/mL

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 68 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

21B1

Isotype

IgG1

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "12.03 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.361 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.361 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EWS protein also known as EWSR1 or 2'1b1' comes from the EWSR1 gene. It is a part of the TET family of proteins and has a molecular weight of about 68 kDa. EWS is expressed widely in human tissues with high levels in the brain gonads and skeletal muscle. The protein contains an RNA-binding domain and amino acid repeats which allow it to interact with other proteins and RNA molecules.
Biological function summary

The EWS protein plays roles in various cellular processes such as transcription and RNA splicing. It can participate in forming different protein complexes which influence its function. EWS serves as a cofactor for several transcription factors and it is important in regulating the expression of genes involved in cell cycle progression and differentiation. The protein’s interaction with RNA and other molecules highlights its multifunctional nature within cellular environments.

Pathways

EWS engages in pathways like the regulation of transcription and RNA processing. It closely associates with proteins involved in gene expression surveillance such as the RNA polymerase II which is significant for transcription activities. EWS partners with proteins such as FUS and TAF15 which are essential parts of transcription regulation networks. These interactions place EWS in a central role in cellular regulatory machinery.

EWS is notably linked to Ewing sarcoma and neurodegenerative diseases. In Ewing sarcoma EWS forms fusion proteins like EWS-FLI1 by translocation events which lead to tumor formation. This fusion protein acts abnormally in transcription regulation driving oncogenic pathways. EWS's interaction with proteins like FUS and TAF15 also relate it to certain neurodegenerative conditions indicating its importance in both cancer and neurobiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Might normally function as a transcriptional repressor (PubMed : 10767297). EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
See full target information EWSR1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1529657 PubMed40510136

2025

METTL3-mediated SNHG1 mA modification promotes proliferation and migration through transcriptional regulation of WDR74 in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guanzhen Qiu,Yuxin Bao,Yuanzhuang Zhang,Yeqiu Xu,Tianhua Qiao,Chenghao Li,Hanjie Zhai,Zhenjun Chen,Fu Ren,Yong Wang

Current protocols 4:e1030 PubMed38923763

2024

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein-Ligand Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Dana M Burris,Samuel W Gillespie,Emma Joy Campbell,S Nick Ice,Vikas Yadav,William D Picking,Christian L Lorson,Kamal Singh

Biomolecules 10: PubMed32664515

2020

Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ashish Kumar-Singh,Jatin Shrinet,Malgorzata Maria Parniewska,Jonas Fuxe,Katalin Dobra,Anders Hjerpe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com