Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (AB255785)

EWSR1/EWS was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252829 at 12.03μg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab252829 IP in HeLa whole cell lysate.
Lane 3 : Rat monoclonal IgG instead of ab252829 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

All lanes:

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (<a href='/en-us/products/primary-antibodies/ewsr1-ews-antibody-21b1-ab252829'>ab252829</a>)

Predicted band size: 68 kDa

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (AB255785)

EWSR1/EWS was immunoprecipitated from K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252829 at 12.03μg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1 : K562 whole cell lysate 10μg (Input).
Lane 2 : ab252829 IP in K562 whole cell lysate.
Lane 3 : Rat monoclonal IgG instead of ab252829 in K562 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

All lanes:

Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (<a href='/en-us/products/primary-antibodies/ewsr1-ews-antibody-21b1-ab252829'>ab252829</a>)

Predicted band size: 68 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (AB255785)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 15 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

All lanes:

Western blot - Anti-EWSR1/EWS antibody [21B1] (<a href='/en-us/products/primary-antibodies/ewsr1-ews-antibody-21b1-ab252829'>ab252829</a>) at 0.361 µg/mL

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 68 kDa

false

Exposure time: 15s