Rabbit Polyclonal EWSR1/EWS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EWSR1 aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Rat | Tested | Expected | Expected |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
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Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
EWS, EWS, EWSR1, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein
Rabbit Polyclonal EWSR1/EWS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EWSR1 aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The EWS protein also known as EWSR1 or 2'1b1' comes from the EWSR1 gene. It is a part of the TET family of proteins and has a molecular weight of about 68 kDa. EWS is expressed widely in human tissues with high levels in the brain gonads and skeletal muscle. The protein contains an RNA-binding domain and amino acid repeats which allow it to interact with other proteins and RNA molecules.
The EWS protein plays roles in various cellular processes such as transcription and RNA splicing. It can participate in forming different protein complexes which influence its function. EWS serves as a cofactor for several transcription factors and it is important in regulating the expression of genes involved in cell cycle progression and differentiation. The protein’s interaction with RNA and other molecules highlights its multifunctional nature within cellular environments.
EWS engages in pathways like the regulation of transcription and RNA processing. It closely associates with proteins involved in gene expression surveillance such as the RNA polymerase II which is significant for transcription activities. EWS partners with proteins such as FUS and TAF15 which are essential parts of transcription regulation networks. These interactions place EWS in a central role in cellular regulatory machinery.
EWS is notably linked to Ewing sarcoma and neurodegenerative diseases. In Ewing sarcoma EWS forms fusion proteins like EWS-FLI1 by translocation events which lead to tumor formation. This fusion protein acts abnormally in transcription regulation driving oncogenic pathways. EWS's interaction with proteins like FUS and TAF15 also relate it to certain neurodegenerative conditions indicating its importance in both cancer and neurobiology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Paraffin embedded mouse testis tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.
10% SDS-PAGE
All lanes: Western blot - Anti-EWSR1/EWS antibody (ab153719) at 1/1000 dilution
Lane 1: HEK-293T whole cell extract at 30 µg
Lane 2: HeLa whole cell extract at 30 µg
Lane 3: HepG2 whole cell extract at 30 µg
Predicted band size: 68 kDa
Paraffin embedded mouse prostate tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.
Paraffin embedded mouse testis tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.
Paraffin embedded rat fore brain tissue stained for EWSR1 using ab153719 at 1/500 dlution in immunohistochemical analysis.
All lanes: Western blot - Anti-EWSR1/EWS antibody (ab153719) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 68 kDa
Immunohistochemical analysis of paraffin-embedded Human cal27 xenograft tissue labeling EWSR1/EWS with ab153719 at 1/100 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EWSR1/EWS with ab153719 at 1/500 dilution.
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