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Rabbit Polyclonal EWSR1/EWS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EWSR1 aa 1-250.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (AB153719), expandable thumbnail
  • Western blot - Anti-EWSR1/EWS antibody (AB153719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (AB153719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (AB153719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (AB153719), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human EWSR1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q01844

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Alternative names

Recommended products

Rabbit Polyclonal EWSR1/EWS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EWSR1 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human EWSR1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q01844
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The EWS protein also known as EWSR1 or 2'1b1' comes from the EWSR1 gene. It is a part of the TET family of proteins and has a molecular weight of about 68 kDa. EWS is expressed widely in human tissues with high levels in the brain gonads and skeletal muscle. The protein contains an RNA-binding domain and amino acid repeats which allow it to interact with other proteins and RNA molecules.

Biological function summary

The EWS protein plays roles in various cellular processes such as transcription and RNA splicing. It can participate in forming different protein complexes which influence its function. EWS serves as a cofactor for several transcription factors and it is important in regulating the expression of genes involved in cell cycle progression and differentiation. The protein’s interaction with RNA and other molecules highlights its multifunctional nature within cellular environments.

Pathways

EWS engages in pathways like the regulation of transcription and RNA processing. It closely associates with proteins involved in gene expression surveillance such as the RNA polymerase II which is significant for transcription activities. EWS partners with proteins such as FUS and TAF15 which are essential parts of transcription regulation networks. These interactions place EWS in a central role in cellular regulatory machinery.

Associated diseases and disorders

EWS is notably linked to Ewing sarcoma and neurodegenerative diseases. In Ewing sarcoma EWS forms fusion proteins like EWS-FLI1 by translocation events which lead to tumor formation. This fusion protein acts abnormally in transcription regulation driving oncogenic pathways. EWS's interaction with proteins like FUS and TAF15 also relate it to certain neurodegenerative conditions indicating its importance in both cancer and neurobiology.

Product promise

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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719)

    Paraffin embedded mouse testis tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Western blot - Anti-EWSR1/EWS antibody (ab153719)

    10% SDS-PAGE

    All lanes: Western blot - Anti-EWSR1/EWS antibody (ab153719) at 1/1000 dilution

    Lane 1: HEK-293T whole cell extract at 30 µg

    Lane 2: HeLa whole cell extract at 30 µg

    Lane 3: HepG2 whole cell extract at 30 µg

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719)

    Paraffin embedded mouse prostate tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719)

    Paraffin embedded mouse testis tissue stained for EWSR1 using ab153719 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719)

    Paraffin embedded rat fore brain tissue stained for EWSR1 using ab153719 at 1/500 dlution in immunohistochemical analysis.

  • Western blot - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Western blot - Anti-EWSR1/EWS antibody (ab153719)

    All lanes: Western blot - Anti-EWSR1/EWS antibody (ab153719) at 1/1000 dilution

    All lanes: A549 whole cell lysate at 30 µg

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody (ab153719)

    Immunohistochemical analysis of paraffin-embedded Human cal27 xenograft tissue labeling EWSR1/EWS with ab153719 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody (ab153719), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody (ab153719)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EWSR1/EWS with ab153719 at 1/500 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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