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AB133318

Anti-EWSR1/EWS antibody [EPR4648]

2

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal EWSR1/EWS antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

EWS, EWSR1, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)

Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling EWSR1/EWS with ab133318 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)

Immunohistochemical analysis of Paraffin Embedded Human spleen tissue labelling EWSR1/EWS with ab133318 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)
  • WB

Unknown

Western blot - Anti-EWSR1/EWS antibody [EPR4648] (AB133318)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-EWSR1/EWS antibody [EPR4648] (ab133318) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

F9 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

false

  • Carrier free

    Anti-EWSR1/EWS antibody [EPR4648] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4648

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EWS protein also known as EWSR1 or 2'1b1' comes from the EWSR1 gene. It is a part of the TET family of proteins and has a molecular weight of about 68 kDa. EWS is expressed widely in human tissues with high levels in the brain gonads and skeletal muscle. The protein contains an RNA-binding domain and amino acid repeats which allow it to interact with other proteins and RNA molecules.
Biological function summary

The EWS protein plays roles in various cellular processes such as transcription and RNA splicing. It can participate in forming different protein complexes which influence its function. EWS serves as a cofactor for several transcription factors and it is important in regulating the expression of genes involved in cell cycle progression and differentiation. The protein’s interaction with RNA and other molecules highlights its multifunctional nature within cellular environments.

Pathways

EWS engages in pathways like the regulation of transcription and RNA processing. It closely associates with proteins involved in gene expression surveillance such as the RNA polymerase II which is significant for transcription activities. EWS partners with proteins such as FUS and TAF15 which are essential parts of transcription regulation networks. These interactions place EWS in a central role in cellular regulatory machinery.

EWS is notably linked to Ewing sarcoma and neurodegenerative diseases. In Ewing sarcoma EWS forms fusion proteins like EWS-FLI1 by translocation events which lead to tumor formation. This fusion protein acts abnormally in transcription regulation driving oncogenic pathways. EWS's interaction with proteins like FUS and TAF15 also relate it to certain neurodegenerative conditions indicating its importance in both cancer and neurobiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Might normally function as a transcriptional repressor (PubMed : 10767297). EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
See full target information EWSR1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 128:1941-1954 PubMed36959380

2023

Functional genomics of human clear cell sarcoma: genomic, transcriptomic and chemical biology landscape for clear cell sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel V Rasmussen,Agnieszka Wozniak,Melvin Lathara,Joshua M Goldenberg,Benjamin M Samudio,Lissett R Bickford,Kiyo Nagamori,Hollis Wright,Andrew D Woods,Shefali Chauhan,Che-Jui Lee,Erin R Rudzinski,Michael K Swift,Tadashi Kondo,David E Fisher,Evgeny Imyanitov,Isidro Machado,Antonio Llombart-Bosch,Irene L Andrulis,Nalan Gokgoz,Jay Wunder,Hiroshi Mirotaki,Takuro Nakamura,Ganapati Srinivasa,Khin Thway,Robin L Jones,Paul H Huang,Noah E Berlow,Patrick Schöffski,Charles Keller

Brain : a journal of neurology 134:2595-609 PubMed21856723

2011

FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations.

Applications

IHC-P

Species

Human

Manuela Neumann,Eva Bentmann,Dorothee Dormann,Ali Jawaid,Mariely DeJesus-Hernandez,Olaf Ansorge,Sigrun Roeber,Hans A Kretzschmar,David G Munoz,Hirofumi Kusaka,Osamu Yokota,Lee-Cyn Ang,Juan Bilbao,Rosa Rademakers,Christian Haass,Ian R A Mackenzie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com