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AB121236

Anti-EXD2 antibody

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(1 Publication)

Rabbit Polyclonal EXD2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EXD2 aa 300-450.

View Alternative Names

C14orf114, EXDL2, EXD2, Exonuclease 3'-5' domain-containing protein 2, 3'-5' exoribonuclease EXD2, Exonuclease 3'-5' domain-like-containing protein 2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)

Immunohistochemical analysis of human kidney tissue labeling exd2 with ab121236. The data shows moderate to strong cytoplasmic positivity in cells in glomeruli.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)

Immunohistochemical analysis of human testis tissue labeling exd2 with ab121236. The data shows moderate to strong cytoplasmic positivity in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-EXD2 antibody (AB121236)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EXD2 antibody (AB121236)

Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121236 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)

Immunohistochemical analysis of human cerebral cortex tissue labeling exd2 with ab121236. The data shows moderate to strong nuclear and cytoplasmic positivity in neurons.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (AB121236)

Immunohistochemical analysis of human prostate tissue labeling exd2 with ab121236. The data shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.

Western blot - Anti-EXD2 antibody (AB121236)
  • WB

Supplier Data

Western blot - Anti-EXD2 antibody (AB121236)

All lanes:

Western blot - Anti-EXD2 antibody (ab121236) at 0.4 µg/mL

All lanes:

A431 cell lysate

Predicted band size: 56 kDa

false

Western blot - Anti-EXD2 antibody (AB121236)
  • WB

Supplier Data

Western blot - Anti-EXD2 antibody (AB121236)

All lanes:

Western blot - Anti-EXD2 antibody (ab121236) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EXD2 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q9NVH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p>pH 6.0</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXD2 also known as Exoribonuclease 2 is an exoribonuclease enzyme with a molecular mass of approximately 72 kDa. It is found mainly in the mitochondria where it helps ensure mitochondrial RNA stability. This enzyme carries out 3'-5' exoribonuclease activity cleaving RNA substrates to maintain proper mitochondrial function. Researchers observe its expression in several tissues with pronounced levels noticed in highly energy-dependent tissues such as muscle and brain where mitochondrial efficiency is important.
Biological function summary

The enzyme plays a significant role in mitochondrial RNA metabolism. EXD2 participates in RNA degradation essential for the integrity of mitochondrial gene expression. It does not function in isolation; instead it forms complexes with other mitochondrial RNA-binding proteins to execute its nuclease activity efficiently. These interactions help regulate the turnover of mitochondrial mRNAs ensuring the correct expression levels for mitochondrial proteins.

Pathways

EXD2 holds an integral position in mitochondrial gene expression and oxidative phosphorylation pathways. Within the oxidative phosphorylation pathway EXD2 assists in regulating RNA components required for the synthesis of core subunits of the electron transport chain. Additionally EXD2 interacts with proteins like POLRMT which is necessary for mitochondrial RNA transcription therefore linking RNA metabolism to energy production.

Researchers link EXD2 to mitochondrial diseases and some neurodegenerative disorders. These conditions can arise when EXD2 is dysregulated leading to defects in energy metabolism due to improper mitochondrial function. Mutations or loss of function in EXD2 may associate with reduced oxidative phosphorylation efficiency and symptoms presenting in disorders such as Leigh syndrome. Furthermore disorders involving mitochondrial dysfunction often involve complex interactions with proteins like OPA1 connecting to broader mitochondrial dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exonuclease that has both 3'-5' exoribonuclease and exodeoxyribonuclease activities, depending on the divalent metal cation used as cofactor (PubMed : 29335528, PubMed : 31127291). In presence of Mg(2+), only shows 3'-5' exoribonuclease activity, while it shows both exoribonuclease and exodeoxyribonuclease activities in presence of Mn(2+) (PubMed : 29335528, PubMed : 31127291). Acts as an exoribonuclease in mitochondrion, possibly by regulating ATP production and mitochondrial translation (PubMed : 29335528). Also involved in the response to DNA damage (PubMed : 26807646, PubMed : 31255466). Acts as 3'-5' exodeoxyribonuclease for double-strand breaks resection and efficient homologous recombination (PubMed : 20603073, PubMed : 26807646). Plays a key role in controlling the initial steps of chromosomal break repair, it is recruited to chromatin in a damage-dependent manner and functionally interacts with the MRN complex to accelerate resection through its 3'-5' exonuclease activity, which efficiently processes double-stranded DNA substrates containing nicks (PubMed : 26807646). Also involved in response to replicative stress : recruited to stalled forks and is required to stabilize and restart stalled replication forks by restraining excessive fork regression, thereby suppressing their degradation (PubMed : 31255466).
See full target information EXD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:13 PubMed38308285

2024

USP40 promotes hepatocellular carcinoma cell proliferation, migration and stemness by deubiquitinating and stabilizing Claudin1.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Wu,Yuanyuan Qiu,Jinhui Guo,Zibo Yuan,Yingnan Yang,Qingwei Zhu,Zhe Zhang,Junwei Guo,Yanfang Wu,Junyu Zhang,Dongsheng Huang,Kangsheng Tu,Xiaoge Hu
View all publications
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Product promise

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