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AB169672

Anti-EXD2 antibody

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(1 Publication)

Mouse Polyclonal EXD2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EXD2.

View Alternative Names

C14orf114, EXDL2, EXD2, Exonuclease 3'-5' domain-containing protein 2, 3'-5' exoribonuclease EXD2, Exonuclease 3'-5' domain-like-containing protein 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-EXD2 antibody (AB169672)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EXD2 antibody (AB169672)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling EXD2 using ab169672 at 10 µg/ml.

Western blot - Anti-EXD2 antibody (AB169672)
  • WB

Unknown

Western blot - Anti-EXD2 antibody (AB169672)

All lanes:

Western blot - Anti-EXD2 antibody (ab169672) at 1 µg/mL

Lane 1:

EXD2-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 56 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EXD2.

Q9NVH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXD2 also known as Exoribonuclease 2 is an exoribonuclease enzyme with a molecular mass of approximately 72 kDa. It is found mainly in the mitochondria where it helps ensure mitochondrial RNA stability. This enzyme carries out 3'-5' exoribonuclease activity cleaving RNA substrates to maintain proper mitochondrial function. Researchers observe its expression in several tissues with pronounced levels noticed in highly energy-dependent tissues such as muscle and brain where mitochondrial efficiency is important.
Biological function summary

The enzyme plays a significant role in mitochondrial RNA metabolism. EXD2 participates in RNA degradation essential for the integrity of mitochondrial gene expression. It does not function in isolation; instead it forms complexes with other mitochondrial RNA-binding proteins to execute its nuclease activity efficiently. These interactions help regulate the turnover of mitochondrial mRNAs ensuring the correct expression levels for mitochondrial proteins.

Pathways

EXD2 holds an integral position in mitochondrial gene expression and oxidative phosphorylation pathways. Within the oxidative phosphorylation pathway EXD2 assists in regulating RNA components required for the synthesis of core subunits of the electron transport chain. Additionally EXD2 interacts with proteins like POLRMT which is necessary for mitochondrial RNA transcription therefore linking RNA metabolism to energy production.

Researchers link EXD2 to mitochondrial diseases and some neurodegenerative disorders. These conditions can arise when EXD2 is dysregulated leading to defects in energy metabolism due to improper mitochondrial function. Mutations or loss of function in EXD2 may associate with reduced oxidative phosphorylation efficiency and symptoms presenting in disorders such as Leigh syndrome. Furthermore disorders involving mitochondrial dysfunction often involve complex interactions with proteins like OPA1 connecting to broader mitochondrial dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exonuclease that has both 3'-5' exoribonuclease and exodeoxyribonuclease activities, depending on the divalent metal cation used as cofactor (PubMed : 29335528, PubMed : 31127291). In presence of Mg(2+), only shows 3'-5' exoribonuclease activity, while it shows both exoribonuclease and exodeoxyribonuclease activities in presence of Mn(2+) (PubMed : 29335528, PubMed : 31127291). Acts as an exoribonuclease in mitochondrion, possibly by regulating ATP production and mitochondrial translation (PubMed : 29335528). Also involved in the response to DNA damage (PubMed : 26807646, PubMed : 31255466). Acts as 3'-5' exodeoxyribonuclease for double-strand breaks resection and efficient homologous recombination (PubMed : 20603073, PubMed : 26807646). Plays a key role in controlling the initial steps of chromosomal break repair, it is recruited to chromatin in a damage-dependent manner and functionally interacts with the MRN complex to accelerate resection through its 3'-5' exonuclease activity, which efficiently processes double-stranded DNA substrates containing nicks (PubMed : 26807646). Also involved in response to replicative stress : recruited to stalled forks and is required to stabilize and restart stalled replication forks by restraining excessive fork regression, thereby suppressing their degradation (PubMed : 31255466).
See full target information EXD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 535:164-8 PubMed27383988

2016

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhang,Jonathan J Miner,Matthew J Gorman,Keiko Rausch,Holly Ramage,James P White,Adam Zuiani,Ping Zhang,Estefania Fernandez,Qiang Zhang,Kimberly A Dowd,Theodore C Pierson,Sara Cherry,Michael S Diamond
View all publications
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