Anti-EXOC2 antibody [EPR9420] - BSA and Azide free

• WB

Unknown

Western blot - Anti-EXOC2 antibody [EPR9420] - BSA and Azide free (AB248907)

This data was developed using ab140620, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EXOC2 antibody [EPR9420] (<a href='/en-us/products/primary-antibodies/exoc2-antibody-epr9420-ab140620'>ab140620</a>) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

Human placenta tissue lysate at 10 µg

Lane 3:

Fetal brain tissue lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 104 kDa

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• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EXOC2 antibody [EPR9420] - BSA and Azide free (AB248907)

This data was developed using ab140620, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labelling EXOC2 with ab140620 at 1/10 dilution (red) or a Rabbit IgG negative control (green).

• WB

Lab

Western blot - Anti-EXOC2 antibody [EPR9420] - BSA and Azide free (AB248907)

This data was developed using the same antibody clone in a different buffer formulation (ab140620).

Lanes 1- 4 : Merged signal (red and green). Green - ab140620 observed at 104 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab140620 was shown to react with EXOC2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab265839 (CRISPR/Cas9 edited cell lysate ab257944) lane below 104kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EXOC2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab140620 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EXOC2 antibody [EPR9420] (<a href='/en-us/products/primary-antibodies/exoc2-antibody-epr9420-ab140620'>ab140620</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EXOC2 CRISPR/Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human EXOC2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-exoc2-knockout-hela-cell-line-ab265839'>ab265839</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 104 kDa

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