AB95981

Anti-EXOC7 antibody

Name: Anti-EXOC7 antibody (ab95981) | Abcam
Brand: Abcam Limited
SKU: AB95981
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal EXOC7 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EXOC7 aa 50-350.

View Alternative Names

EXO70, KIAA1067, EXOC7, Exocyst complex component 7, Exocyst complex component Exo70

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EXOC7 antibody (AB95981)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EXOC7 antibody (AB95981)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab95981 at 1/200 dilution. Lower image merged with DNA probe.

Unknown

Western blot - Anti-EXOC7 antibody (AB95981)

7.5% SDS PAGE

All lanes:

Western blot - Anti-EXOC7 antibody (ab95981) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 83 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EXOC7 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9UPT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXOC7 also known as Exocyst Complex Component 7 is a part of the exocyst complex involved in the regulation of vesicle trafficking. This protein weighs approximately 76 kDa and functions by facilitating the fusion of vesicles with the plasma membrane. It is generally expressed in various tissue types but shows higher levels in lung and brain tissues. EXOC7 often works with other components of the exocyst complex to carry out its role in cellular trafficking.

Biological function summary

EXOC7 plays a significant role in exocyst-mediated processes acting as a critical component of the exocyst complex. This multi-protein complex is essential for targeting secretory vesicles to specific docking sites in the plasma membrane. EXOC7 is involved in processes like cell polarity migration and even in the maintenance of tight junctions. Through its interactions with other exocyst components it aids in the spatial organization of the cell influencing overall cellular architecture and functionality.

Pathways

EXOC7 integrates into exocytosis and cell signaling pathways. It particularly plays a role in the exocytosis pathway which is essential for secretion and cell-surface receptor recycling. EXOC7 interacts with other exocyst complex proteins such as SEC10 and SEC15 promoting efficient vesicle tethering and fusion events. Additionally it links to the ARF6-dependent pathways that affect actin cytoskeleton remodeling demonstrating its importance in cell movement and structural remodeling.

EXOC7's proper function is essential for normal cellular processes and its dysregulation has connections to type 2 diabetes and certain cancers. In type 2 diabetes misregulated exocyst complex activities including those involving EXOC7 can disrupt insulin secretion. In cancer altered expression or function of the exocyst complex may contribute to metastasis due to its role in cell adhesion and migration. EXOC7's interaction with other proteins such as EXOC3L1 and EXOC5 could play a significant role in these disease mechanisms highlighting its potential as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion (By similarity). It is required for neuron survival and plays an essential role in cortical development (By similarity).

See full target information EXOC7

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:327 PubMed39716292

2024

Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Guanjin Shi,Yanyan Ge,Shanshan Huang,Ningning Cui,Le Tan,Rui Liu,Xuefeng Yang

Life science alliance 8: PubMed39448266

2024

SNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Cabrera-Reyes,Teemly Contreras-Palacios,Romina Ulloa,Jorge Jara-Wilde,Mia Caballero,Clara Quiroga,Carmen G Feijoo,Jheimmy Díaz-Muñoz,María-Isabel Yuseff

eLife 11: PubMed36111670

2022

Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Judith Pineau,Léa Pinon,Olivier Mesdjian,Jacques Fattaccioli,Ana-Maria Lennon Duménil,Paolo Pierobon

Frontiers in immunology 12:801164 PubMed35222354

2022

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Ulloa,Oreste Corrales,Fernanda Cabrera-Reyes,Jorge Jara-Wilde,Juan José Saez,Christopher Rivas,Jonathan Lagos,Steffen Härtel,Clara Quiroga,María-Isabel Yuseff,Jheimmy Diaz-Muñoz

The Journal of cell biology 218:2247-2264 PubMed31197029

2019

The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan José Sáez,Jheimmy Diaz,Jorge Ibañez,Juan Pablo Bozo,Fernanda Cabrera Reyes,Martina Alamo,François-Xavier Gobert,Dorian Obino,María Rosa Bono,Ana-María Lennon-Duménil,Charles Yeaman,María-Isabel Yuseff

The Journal of cell biology 217:3863-3872 PubMed30275106

2018

remodels the plasma membrane-derived vacuole by utilizing exocyst components as tethers.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Arasaki,Hana Kimura,Mitsuo Tagaya,Craig R Roy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EXOC7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EXOC7 antibody (AB95981)

Western blot - Anti-EXOC7 antibody (AB95981)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise