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AB155553

Anti-Exonuclease 1 antibody

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(2 Publications)

Rabbit Polyclonal Exonuclease 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EXO1 aa 450-800.

View Alternative Names

EXOI, HEX1, EXO1, Exonuclease 1, hExo1, Exonuclease I, hExoI

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Exonuclease 1 antibody (AB155553)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Exonuclease 1 antibody (AB155553)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling Exonuclease 1 with ab155553 at 1/500 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

Western blot - Anti-Exonuclease 1 antibody (AB155553)
  • WB

Unknown

Western blot - Anti-Exonuclease 1 antibody (AB155553)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 94 kDa

false

Western blot - Anti-Exonuclease 1 antibody (AB155553)
  • WB

Unknown

Western blot - Anti-Exonuclease 1 antibody (AB155553)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution

All lanes:

Mouse thymus whole cell lysate at 50 µg

Predicted band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EXO1 aa 450-800. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ84

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exonuclease 1 (Exo1) also known simply as Exonuclease I is a protein playing a significant mechanical role in DNA processes. The protein functions by degrading nucleic acid molecules from their ends specifically acting on the 5' ends to progressively remove nucleotides. Exo1 exhibits specificity for double-stranded DNA and participates mainly in DNA repair and recombination. The molecular weight of Exo1 is approximately 94 kDa. Expression of Exo1 occurs in various tissues with prevalent expression in tissues undergoing active cell division.
Biological function summary

Exo1 is key in maintaining genomic stability. This protein engages in homologous recombination mismatch repair and the resection process during DNA double-strand break repair. Exo1 works collaboratively as part of larger complexes particularly interacting with proteins like MRE11-RAD50-NBS1 (MRN) complex in DNA repair mechanisms. The interaction within these complexes enhances its efficacy in processing DNA ends and facilitates accurate repair.

Pathways

Exo1 is integral to the DNA repair and homologous recombination pathways. In the DNA repair pathway Exo1 functions in conjunction with proteins such as MutL homologs and DNA polymerases ensuring proper mismatch repair by creating single-strand gaps. Through homologous recombination Exo1 links with proteins such as BRCA1 and RAD51 facilitating the repair of double-strand breaks by processing the DNA ends to allow strand invasion and exchange steps of recombination.

Exo1 has connections with cancer and hereditary nonpolyposis colorectal cancer (HNPCC). In cancer impaired or mutated Exo1 can lead to defective DNA repair mechanisms resulting in genomic instability and tumorigenesis. The protein shows linkage with MLH1 in HNPCC as both participate in mismatch repair. Faulty interactions or mutations in either Exo1 or associated proteins like MLH1 or MSH2 can contribute to the pathogenesis of these conditions highlighting the gene-disease relationship within the context of DNA repair processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
See full target information EXO1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 55:842-852 PubMed37227155

2023

The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Panpan Huang,Yanli Li,Wenyu Liu,Jing Li,Rui Zhao,Haihua Feng,Ce Shi,Gaolu Cao

Cells 9: PubMed33291797

2020

Ca1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Moreno,Tamara Hermosilla,Paulina Hardy,Víctor Aballai,Patricio Rojas,Diego Varela
View all publications
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Product promise

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