AB95012

Anti-Exonuclease 1 antibody

Name: Anti-Exonuclease 1 antibody (ab95012) | Abcam
Brand: Abcam Limited
SKU: AB95012

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(6 Publications)

Rabbit Polyclonal Exonuclease 1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EXO1 aa 700 to C-terminus.

View Alternative Names

EXOI, HEX1, EXO1, Exonuclease 1, hExo1, Exonuclease I, hExoI

2 Images

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Immunoprecipitation - Anti-Exonuclease 1 antibody (AB95012)

ab95012 at 1 µg/ml detecting Exonuclease 1 in HeLa whole cell lysate by WB following IP.
Lane 1 : ab95012 with 10µg/mg of lysate
Lane 2 : IP with an antibody which recognizes an downstream epitope of Exonuclease 1
Lane 3 : control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-Exonuclease 1 antibody (ab95012)

Predicted band size: 94 kDa

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Unknown

Western blot - Anti-Exonuclease 1 antibody (AB95012)

All lanes:

Western blot - Anti-Exonuclease 1 antibody (ab95012) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 94 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EXO1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ84

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab95012 was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exonuclease 1 (Exo1) also known simply as Exonuclease I is a protein playing a significant mechanical role in DNA processes. The protein functions by degrading nucleic acid molecules from their ends specifically acting on the 5' ends to progressively remove nucleotides. Exo1 exhibits specificity for double-stranded DNA and participates mainly in DNA repair and recombination. The molecular weight of Exo1 is approximately 94 kDa. Expression of Exo1 occurs in various tissues with prevalent expression in tissues undergoing active cell division.

Biological function summary

Exo1 is key in maintaining genomic stability. This protein engages in homologous recombination mismatch repair and the resection process during DNA double-strand break repair. Exo1 works collaboratively as part of larger complexes particularly interacting with proteins like MRE11-RAD50-NBS1 (MRN) complex in DNA repair mechanisms. The interaction within these complexes enhances its efficacy in processing DNA ends and facilitates accurate repair.

Pathways

Exo1 is integral to the DNA repair and homologous recombination pathways. In the DNA repair pathway Exo1 functions in conjunction with proteins such as MutL homologs and DNA polymerases ensuring proper mismatch repair by creating single-strand gaps. Through homologous recombination Exo1 links with proteins such as BRCA1 and RAD51 facilitating the repair of double-strand breaks by processing the DNA ends to allow strand invasion and exchange steps of recombination.

Exo1 has connections with cancer and hereditary nonpolyposis colorectal cancer (HNPCC). In cancer impaired or mutated Exo1 can lead to defective DNA repair mechanisms resulting in genomic instability and tumorigenesis. The protein shows linkage with MLH1 in HNPCC as both participate in mismatch repair. Faulty interactions or mutations in either Exo1 or associated proteins like MLH1 or MSH2 can contribute to the pathogenesis of these conditions highlighting the gene-disease relationship within the context of DNA repair processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.

See full target information EXO1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Animal cells and systems 29:502-511 PubMed40771437

2025

Targeting MMR-deficient colorectal cancer with a potent small molecule UNI110.

Applications

Unspecified application

Species

Unspecified reactive species

Enkhzul Amarsanaa,Jung-Min Oh,Seon Young Lee,Saikat Maiti,Sung You Hong,Kyungjae Myung

Nucleic acids research 51:5584-5602 PubMed37140056

2023

MSH2-MSH3 promotes DNA end resection during homologous recombination and blocks polymerase theta-mediated end-joining through interaction with SMARCAD1 and EXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Min Oh,Yujin Kang,Jumi Park,Yubin Sung,Dayoung Kim,Yuri Seo,Eun A Lee,Jae Sun Ra,Enkhzul Amarsanaa,Young-Un Park,Seon Young Lee,Jung Me Hwang,Hongtae Kim,Orlando Schärer,Seung Woo Cho,Changwook Lee,Kei-Ichi Takata,Ja Yil Lee,Kyungjae Myung

Cell death discovery 7:260 PubMed34552057

2021

ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Yan,Man Song,Jie Ping,Shu-Ting Lai,Xiao-Yu Cao,Chen-Jun Bai,Da-Fei Xie,Hua Guan,Shan-Shan Gao,Ping-Kun Zhou

Journal of Cancer 11:4917-4932 PubMed32626539

2020

Plays a Carcinogenic Role in Hepatocellular Carcinoma and is related to the regulation of .

Applications

Unspecified application

Species

Unspecified reactive species

Guang Yang,Keshuai Dong,Zunyi Zhang,Erlei Zhang,Binyong Liang,Xiaoping Chen,Zhiyong Huang

Cell death and differentiation 27:1383-1397 PubMed31562368

2019

RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xie,Yi-Ke Liu,Zong-Pei Guo,Hua Guan,Xiao-Dan Liu,Da-Fei Xie,Yi-Guo Jiang,Teng Ma,Ping-Kun Zhou

Nature communications 6:7744 PubMed26215093

2015

MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.

Applications

Species

Unspecified reactive species

Kyung Yong Lee,Jun-Sub Im,Etsuko Shibata,Jonghoon Park,Naofumi Handa,Stephen C Kowalczykowski,Anindya Dutta

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Exonuclease 1 antibody

Immunoprecipitation - Anti-Exonuclease 1 antibody (AB95012)

Western blot - Anti-Exonuclease 1 antibody (AB95012)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise