AB95068

Anti-Exonuclease 1 antibody

Name: Anti-Exonuclease 1 antibody (ab95068) | Abcam
Brand: Abcam Limited
SKU: AB95068
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal Exonuclease 1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human EXO1 aa 750 to C-terminus.

View Alternative Names

EXOI, HEX1, EXO1, Exonuclease 1, hExo1, Exonuclease I, hExoI

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Immunoprecipitation - Anti-Exonuclease 1 antibody (AB95068)

ab95068 at 0.4 µg/ml detecting Exonuclease 1 in HeLa whole cell lysate by WB following IP.
Lane 1 : IP with an antibody which recognizes an upstream epitope of Exonuclease 1
Lane 2 : ab95068 at 10µg/mg of lysate
Lane 3 : control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-Exonuclease 1 antibody (ab95068)

Predicted band size: 94 kDa

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Unknown

Western blot - Anti-Exonuclease 1 antibody (AB95068)

All lanes:

Western blot - Anti-Exonuclease 1 antibody (ab95068) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 94 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EXO1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ84

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab95068 was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exonuclease 1 (Exo1) also known simply as Exonuclease I is a protein playing a significant mechanical role in DNA processes. The protein functions by degrading nucleic acid molecules from their ends specifically acting on the 5' ends to progressively remove nucleotides. Exo1 exhibits specificity for double-stranded DNA and participates mainly in DNA repair and recombination. The molecular weight of Exo1 is approximately 94 kDa. Expression of Exo1 occurs in various tissues with prevalent expression in tissues undergoing active cell division.

Biological function summary

Exo1 is key in maintaining genomic stability. This protein engages in homologous recombination mismatch repair and the resection process during DNA double-strand break repair. Exo1 works collaboratively as part of larger complexes particularly interacting with proteins like MRE11-RAD50-NBS1 (MRN) complex in DNA repair mechanisms. The interaction within these complexes enhances its efficacy in processing DNA ends and facilitates accurate repair.

Pathways

Exo1 is integral to the DNA repair and homologous recombination pathways. In the DNA repair pathway Exo1 functions in conjunction with proteins such as MutL homologs and DNA polymerases ensuring proper mismatch repair by creating single-strand gaps. Through homologous recombination Exo1 links with proteins such as BRCA1 and RAD51 facilitating the repair of double-strand breaks by processing the DNA ends to allow strand invasion and exchange steps of recombination.

Exo1 has connections with cancer and hereditary nonpolyposis colorectal cancer (HNPCC). In cancer impaired or mutated Exo1 can lead to defective DNA repair mechanisms resulting in genomic instability and tumorigenesis. The protein shows linkage with MLH1 in HNPCC as both participate in mismatch repair. Faulty interactions or mutations in either Exo1 or associated proteins like MLH1 or MSH2 can contribute to the pathogenesis of these conditions highlighting the gene-disease relationship within the context of DNA repair processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.

See full target information EXO1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8476 PubMed41006228

2025

EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Maric,Sandra Segura-Bayona,Raviprasad Kuthethur,Tohru Takaki,Valerie Borel,Tyler H Stanage,Miroslav P Ivanov,Nishita Parnandi,Graeme Hewitt,Rhona Millar,Carmen S Fonseca,Harshil Patel,Miriam Llorian,Scott Warchal,Michael Howell,Arnab Ray Chaudhuri,Panagiotis Kotsantis,Simon J Boulton

Nature 634:492-500 PubMed39261728

2024

Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Ceppi,Maria Rosaria Dello Stritto,Martin Mütze,Stefan Braunshier,Valentina Mengoli,Giordano Reginato,Hồ Mỹ Phúc Võ,Sonia Jimeno,Ananya Acharya,Megha Roy,Aurore Sanchez,Swagata Halder,Sean Michael Howard,Raphaël Guérois,Pablo Huertas,Sylvie M Noordermeer,Ralf Seidel,Petr Cejka

Cell reports 42:112792 PubMed37454295

2023

ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy Leung,Antoine Simoneau,Sneha Saxena,Jessica Jackson,Parasvi S Patel,Mangsi Limbu,Alessandro Vindigni,Lee Zou

Genes & development 37:119-135 PubMed36746606

2023

PLK1 regulates CtIP and DNA2 interplay in long-range DNA end resection.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Ceppi,Elda Cannavo,Hélène Bret,Rosa Camarillo,Francesca Vivalda,Roshan Singh Thakur,Amador Romero-Franco,Alessandro A Sartori,Pablo Huertas,Raphaël Guérois,Petr Cejka

Nature communications 13:5295 PubMed36075911

2022

Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Bert van de Kooij,Alex Kruswick,Haico van Attikum,Michael B Yaffe

Science translational medicine 14:eabc7480 PubMed35353542

2022

Small-molecule targeted therapies induce dependence on DNA double-strand break repair in residual tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Moiez Ali,Min Lu,Hazel Xiaohui Ang,Ryan S Soderquist,Christine E Eyler,Haley M Hutchinson,Carolyn Glass,Christopher F Bassil,Omar M Lopez,D Lucas Kerr,Christina J Falcon,Helena A Yu,Aaron N Hata,Collin M Blakely,Caroline E McCoach,Trever G Bivona,Kris C Wood

Nature communications 12:6561 PubMed34772932

2021

WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arindam Datta,Kajal Biswas,Joshua A Sommers,Haley Thompson,Sanket Awate,Claudia M Nicolae,Tanay Thakar,George-Lucian Moldovan,Robert H Shoemaker,Shyam K Sharan,Robert M Brosh

Neoplasma 68:1147-1156 PubMed34427100

2021

SLC7A11 negatively associates with mismatch repair gene expression and endows glioblastoma cells sensitive to radiation under low glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Hu,Wei-Hua Hu,Shao-Long Zhou,Zhuo Yang,Wu-Long Liang,Ru-Yi Yang,Ming-He Li,Zhou Jing,Zi-An Li,Xu-Dong Fu,Xin-Jun Wang

PloS one 15:e0235998 PubMed33253193

2020

Replication stress and FOXM1 drive radiation induced genomic instability and cell transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhentian Li,David S Yu,Paul W Doetsch,Erica Werner

Aging 12:9549-9584 PubMed32454462

2020

MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture risk and bone health in postmenopausal diabetic women.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Sheng Chen,Xue-Ran Kang,Zi-Hui Zhou,Jiang Yang,Qi Xin,Chen-Ting Ying,Yun-Peng Zhang,Jie Tao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Exonuclease 1 antibody

Immunoprecipitation - Anti-Exonuclease 1 antibody (AB95068)

Western blot - Anti-Exonuclease 1 antibody (AB95068)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise