Rabbit Polyclonal Exopolyphosphatase antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | |
---|---|
Recombinant full length protein - Saccharomyces cerevisiae | Tested |
Saccharomyces cerevisiae | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Saccharomyces cerevisiae | Dilution info 1/500 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Polyphosphatase (polyPase) involved in the degradation of inorganic polyphosphates (polyP) that is able to degrade a range of chains from three to several hundreds of residues in a highly processive manner (PubMed:7860598). Exclusively shows exopolyphosphatase activity, cleaving inside the polyP chain (PubMed:31175919).
YHR201C, PPX1, Polyphosphatase, PolyPase, Exopolyphosphatase, Metaphosphatase
Rabbit Polyclonal Exopolyphosphatase antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein G
Purity >95%.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Exopolyphosphatase often referred to as PPX functions to degrade inorganic polyphosphates by cleaving terminal phosphate residues. This enzyme facilitates the hydrolysis of polyphosphate chains resulting in shorter phosphates and inorganic phosphate. With a molecular mass around 64 kDa exopolyphosphatase locates itself mainly in the cytoplasm. Studies report its expression in a variety of organisms including bacteria yeast and mammals where it plays a significant role in phosphate metabolism.
Exopolyphosphatase helps regulate intracellular levels of inorganic polyphosphate which acts as a reservoir of energy and phosphate involved in stress response and cellular processes like cation sequestration. Exopolyphosphatase can act as part of larger enzymatic complexes functioning with other polyphosphate-related enzymes. This regulatory function is important for maintaining cellular homoeostasis and supports different cellular responses.
Exopolyphosphatase plays an important role in phosphate turnover and energy metabolism pathways. It influences the synthesis and breakdown of polyphosphate chains which ties into the phosphate regulon and other metabolic pathways related to phosphate economy. It works in association with proteins like polyphosphate kinase which synthesizes polyphosphates creating a balance within the phosphate regulatory network.
Altered exopolyphosphatase activity can link to bacterial pathogenesis and human disorders of phosphate metabolism. Disruptions in its function associate with conditions like osteochondrodysplasia due to imbalanced phosphate levels affecting bone metabolism. Additionally its activity impacts proteins involved in infection processes such as those affecting bacterial virulence making it a target for antimicrobial research.
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All lanes: Western blot - Anti-Exopolyphosphatase antibody (ab225684) at 1/500 dilution
Lane 1: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.06 µg
Lane 2: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.03 µg
Lane 3: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.015 µg
Lane 4: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.0075 µg
All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 46 kDa
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