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Rabbit Polyclonal Exopolyphosphatase antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1.

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Images

Western blot - Anti-Exopolyphosphatase antibody (AB225684), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1. Database link P38698

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Recombinant full length protein - Saccharomyces cerevisiae
Tested
Saccharomyces cerevisiae
Predicted

Tested
Tested

Species

Recombinant full length protein - Saccharomyces cerevisiae

Dilution info

1/500 - 1/5000

Notes

-

Predicted
Predicted

Species

Saccharomyces cerevisiae

Dilution info

-

Notes

-

Target data

Function

Polyphosphatase (polyPase) involved in the degradation of inorganic polyphosphates (polyP) that is able to degrade a range of chains from three to several hundreds of residues in a highly processive manner (PubMed:7860598). Exclusively shows exopolyphosphatase activity, cleaving inside the polyP chain (PubMed:31175919).

Alternative names

Recommended products

Rabbit Polyclonal Exopolyphosphatase antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C PPX1. Database link P38698
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purity >95%.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Exopolyphosphatase often referred to as PPX functions to degrade inorganic polyphosphates by cleaving terminal phosphate residues. This enzyme facilitates the hydrolysis of polyphosphate chains resulting in shorter phosphates and inorganic phosphate. With a molecular mass around 64 kDa exopolyphosphatase locates itself mainly in the cytoplasm. Studies report its expression in a variety of organisms including bacteria yeast and mammals where it plays a significant role in phosphate metabolism.

Biological function summary

Exopolyphosphatase helps regulate intracellular levels of inorganic polyphosphate which acts as a reservoir of energy and phosphate involved in stress response and cellular processes like cation sequestration. Exopolyphosphatase can act as part of larger enzymatic complexes functioning with other polyphosphate-related enzymes. This regulatory function is important for maintaining cellular homoeostasis and supports different cellular responses.

Pathways

Exopolyphosphatase plays an important role in phosphate turnover and energy metabolism pathways. It influences the synthesis and breakdown of polyphosphate chains which ties into the phosphate regulon and other metabolic pathways related to phosphate economy. It works in association with proteins like polyphosphate kinase which synthesizes polyphosphates creating a balance within the phosphate regulatory network.

Associated diseases and disorders

Altered exopolyphosphatase activity can link to bacterial pathogenesis and human disorders of phosphate metabolism. Disruptions in its function associate with conditions like osteochondrodysplasia due to imbalanced phosphate levels affecting bone metabolism. Additionally its activity impacts proteins involved in infection processes such as those affecting bacterial virulence making it a target for antimicrobial research.

Product promise

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1 product image

  • Western blot - Anti-Exopolyphosphatase antibody (ab225684), expandable thumbnail

    Western blot - Anti-Exopolyphosphatase antibody (ab225684)

    All lanes: Western blot - Anti-Exopolyphosphatase antibody (ab225684) at 1/500 dilution

    Lane 1: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.06 µg

    Lane 2: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.03 µg

    Lane 3: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.015 µg

    Lane 4: Recombinant Saccharomyces cerevisiae Exopolyphosphatase protein at 0.0075 µg

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 45 kDa

    Observed band size: 46 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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