Rabbit Polyclonal EXOSC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus.
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Publications
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- Nature communications 13:56092022Human centromere repositioning activates transcription and opens chromatin fibre structure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine Naughton,Covadonga Huidobro,Claudia R Catacchio,Adam Buckle,Graeme R Grimes,Ryu-Suke Nozawa,Stefania Purgato,Mariano Rocchi,Nick GilbertPubMed 36153345
- Nature structural & molecular biology 28:337-3462021A first exon termination checkpoint preferentially suppresses extragenic transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiv M I Austenaa,Viviana Piccolo,Marta Russo,Elena Prosperini,Sara Polletti,Danilo Polizzese,Serena Ghisletti,Iros Barozzi,Giuseppe R Diaferia,Gioacchino NatoliPubMed 33767452
- Molecular cell 77:1340-1349.e62020Extraction of mRNA from Stalled Ribosomes by the Ski Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Zinoviev et. al.PubMed 32006463
- Cell reports 26:2779-2791.e52019Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLee Davidson,Laura Francis,Ross A Cordiner,Joshua D Eaton,Chris Estell,Sara Macias,Javier F Cáceres,Steven WestPubMed 30840897
- Cell 169:679-692.e142017The RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis and Infectivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Rialdi,Judd Hultquist,David Jimenez-Morales,Zuleyma Peralta,Laura Campisi,Romain Fenouil,Natasha Moshkina,Zhen Zhen Wang,Brice Laffleur,Robyn M Kaake,Michael J McGregor,Kelsey Haas,Evangelos Pefanis,Randy A Albrecht,Lars Pache,Sumit Chanda,Joanna Jen,Jordi Ochando,Minji Byun,Uttiya Basu,Adolfo García-Sastre,Nevan Krogan,Harm van Bakel,Ivan MarazziPubMed 28475896
- eLife 5:2016Exosome complex orchestrates developmental signaling to balance proliferation and differentiation during erythropoiesis.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQT5
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Common marmoset | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.
Alternative names
RRP40, CGI-102, EXOSC3, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10
Recommended products
Rabbit Polyclonal EXOSC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQT5
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab156683 was affinity purified using an epitope specific to EXOSC3 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.
- Biological function summary
EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.
- Pathways
The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.
- Associated diseases and disorders
Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EXOSC3 antibody (ab156683)
All lanes: Western blot - Anti-EXOSC3 antibody (ab156683) at 0.1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 30s
Immunoprecipitation - Anti-EXOSC3 antibody (ab156683)
Detection of EXOSC3 by Western blot of Immunoprecipitate.
Immunoprecipitation of 293T whole cell lysate using ab156683 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of EXOSC3 with ab156683 at 1 µg/ml.
The lysate in lane 2 is precipitated with an IgG control antibody.
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-EXOSC3 antibody (ab156683)
Predicted band size: 30 kDa
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