AB156683

Anti-EXOSC3 antibody

Name: Anti-EXOSC3 antibody (ab156683) | Abcam
Brand: Abcam Limited
SKU: AB156683
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal EXOSC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus.

View Alternative Names

RRP40, CGI-102, EXOSC3, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10

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Immunoprecipitation - Anti-EXOSC3 antibody (AB156683)

Detection of EXOSC3 by Western blot of Immunoprecipitate.
Immunoprecipitation of 293T whole cell lysate using ab156683 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of EXOSC3 with ab156683 at 1 µg/ml.
The lysate in lane 2 is precipitated with an IgG control antibody.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-EXOSC3 antibody (ab156683)

Predicted band size: 30 kDa

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Western blot - Anti-EXOSC3 antibody (AB156683)

All lanes:

Western blot - Anti-EXOSC3 antibody (ab156683) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 30 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EXOSC3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NQT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab156683 was affinity purified using an epitope specific to EXOSC3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.

Biological function summary

EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.

Pathways

The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.

Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.

See full target information EXOSC3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5609 PubMed36153345

2022

Human centromere repositioning activates transcription and opens chromatin fibre structure.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Naughton,Covadonga Huidobro,Claudia R Catacchio,Adam Buckle,Graeme R Grimes,Ryu-Suke Nozawa,Stefania Purgato,Mariano Rocchi,Nick Gilbert

Nature structural & molecular biology 28:337-346 PubMed33767452

2021

A first exon termination checkpoint preferentially suppresses extragenic transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Liv M I Austenaa,Viviana Piccolo,Marta Russo,Elena Prosperini,Sara Polletti,Danilo Polizzese,Serena Ghisletti,Iros Barozzi,Giuseppe R Diaferia,Gioacchino Natoli

Molecular cell 77:1340-1349.e6 PubMed32006463

2020

Extraction of mRNA from Stalled Ribosomes by the Ski Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Zinoviev,Rustam K Ayupov,Irina S Abaeva,Christopher U T Hellen,Tatyana V Pestova

Cell reports 26:2779-2791.e5 PubMed30840897

2019

Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control.

Applications

Unspecified application

Species

Unspecified reactive species

Lee Davidson,Laura Francis,Ross A Cordiner,Joshua D Eaton,Chris Estell,Sara Macias,Javier F Cáceres,Steven West

Cell 169:679-692.e14 PubMed28475896

2017

The RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis and Infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Rialdi,Judd Hultquist,David Jimenez-Morales,Zuleyma Peralta,Laura Campisi,Romain Fenouil,Natasha Moshkina,Zhen Zhen Wang,Brice Laffleur,Robyn M Kaake,Michael J McGregor,Kelsey Haas,Evangelos Pefanis,Randy A Albrecht,Lars Pache,Sumit Chanda,Joanna Jen,Jordi Ochando,Minji Byun,Uttiya Basu,Adolfo García-Sastre,Nevan Krogan,Harm van Bakel,Ivan Marazzi

eLife 5: PubMed27543448

2016

Exosome complex orchestrates developmental signaling to balance proliferation and differentiation during erythropoiesis.

Applications

Species

Unspecified reactive species

Skye C McIver,Koichi R Katsumura,Elsa Davids,Peng Liu,Yoon-A Kang,David Yang,Emery H Bresnick

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EXOSC3 antibody

Immunoprecipitation - Anti-EXOSC3 antibody (AB156683)

Western blot - Anti-EXOSC3 antibody (AB156683)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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